FastQCFastQC Report
Wed 25 May 2016
SRR1294919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659718
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60480.1297932621673672No Hit
CCCATGTACTCTGCGTTGATACCAC59250.12715361745066978No Hit
GTATCAACGCAGAGTACTTTTTTTT54370.11668088068848802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA550.00306557212.0932534
CGCGATC750.002652799510.13170717
TTAGACT3950.09.3815664
GTATTAG6550.09.297831
AGCACCG1651.06656444E-79.2125225
GTCTAAG3250.09.076561
GTGTACG1304.2884727E-58.7837671
TAGACAG6650.08.7146655
CTTAGAC3200.08.610863
TAGGACA5000.08.5516584
GTTCTAG5150.08.4995031
GTATAAA9550.08.3698721
GTATTAA10850.08.3317611
GTGTTAG5050.08.2909481
GTATACG1150.00109321388.2745631
GGCAACG1150.00111353068.2591887
GTATCAA58450.08.1889151
CCTATAC3600.08.1819673
CTAATAC4300.08.1758293
GTCTAGG4200.08.1563551
Produced by FastQC (version 0.11.5)