FastQCFastQC Report
Wed 25 May 2016
SRR1294919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659718
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT150640.3232813659539054No Hit
GGTATCAACGCAGAGTACTTTTTTT93460.20057007741670205No Hit
TATCAACGCAGAGTACTTTTTTTTT84120.18052594599072302No Hit
CCCATGTACTCTGCGTTGATACCAC62710.13457895949926585No Hit
GTACATGGGAAGCAGTGGTATCAAC61350.13166032794259222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG1451.6188096E-79.8279145
CCGGACT1252.755624E-59.1203044
CTAATAC5350.08.8789913
GTATTAA9700.08.7219331
TAACGCT1201.764544E-48.7086244
TAATACT8550.08.6669564
GTATCAA75750.08.6337661
GGTATCA45050.08.588041
GCGTGCG1554.215901E-68.580019
GTCTAGG4450.08.5446541
CTTACAC6250.08.5124663
ACTACCG1356.801175E-58.4447265
TAGGCGT1252.7443687E-48.3602796
GTCTTAT4100.08.3466681
GTATTAT8000.08.3176861
TATTACG1150.0011092728.2623862
CGTAATT1150.00111210428.26025916
TAGGACA4750.08.20027354
GTCTAAG3250.08.1897221
GTCTTAG3950.08.1823181
Produced by FastQC (version 0.11.5)