FastQCFastQC Report
Wed 25 May 2016
SRR1294918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994994
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC75790.18971242510001268No Hit
CCCATGTACTCTGCGTTGATACCAC71560.17912417390364038No Hit
GTATCAACGCAGAGTACTTTTTTTT41960.10503144685573995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG658.03968E-411.6903279
ACCGTTC803.7789444E-410.68582258
CACCGTT951.6478969E-49.9984317
GTACGAC800.00451504869.500773
CACCGCG1554.2697866E-79.19291217
GTACCGG950.00180355679.0115691
GTCTTAG4450.08.9778181
CTAATAC5300.08.9629923
GCGCGGT850.00744783468.93988613
GCGCTTG850.00744783468.93988613
GTCCTAG4150.08.9391871
GTGTAAG6000.08.8780641
ACCGCGC1502.7037458E-68.86683118
GTATTAA7050.08.7701241
GTCTAAT3850.08.6474651
TAACGGT1000.00290499448.5504794
GTCTAAG3500.08.4251021
TAATACT6100.08.4103084
GTACTAG2159.509677E-98.406141
AGGACTG6850.08.3212185
Produced by FastQC (version 0.11.5)