FastQCFastQC Report
Wed 25 May 2016
SRR1294918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3994994
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT119280.2985736649416745No Hit
GTACATGGGAAGCAGTGGTATCAAC78040.19534447360872131No Hit
GGTATCAACGCAGAGTACTTTTTTT77190.1932168108387647No Hit
CCCATGTACTCTGCGTTGATACCAC76870.1924158083841928No Hit
TATCAACGCAGAGTACTTTTTTTTT67430.16878623597432185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT550.00306910912.0913244
CTTACCG550.003071699312.0899629
TATACCG1054.1076157E-59.9525975
ACCGACC1054.1121373E-59.9516018
TAGACAG6650.09.5716365
CGGGACT1304.2410793E-69.5003264
ATACCGT900.00111409849.5002066
CCGCGTG800.00452099149.4992559
TACCGTG1201.7035412E-59.4992557
TTATACT7000.09.3646064
GGACCGA1356.8519603E-69.1483476
GACCGTC850.00744381038.9404757
GATAGCG850.00744381038.9404757
CTAGGAC4400.08.8529083
TAGGACG1201.7643595E-48.7086324
AGACCGT1000.0029058988.5501856
AGGACCG1356.8013425E-58.4446295
GTCTTAC4800.08.3173661
TAGTACT5150.08.3012564
AGACCGA1150.00111096418.2610496
Produced by FastQC (version 0.11.5)