FastQCFastQC Report
Wed 25 May 2016
SRR1294917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552252
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38270.10773447379296289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC750.00264851410.1335592
TACGACA800.00451703149.5002124
TGTACCG900.00111460259.4996765
CCCGTGT800.0045249139.4982038
GTATTAG4550.09.4076121
GTATAGA5550.09.2550561
GTCCTAA3400.09.2323721
GGTATCA19150.09.040261
GTCTACG850.00736115878.9526031
ATTATAC6950.08.7483963
TAGGACC2401.0186341E-108.7085274
TAGACAG5450.08.5409945
GTATTAA6850.08.4706661
GTCCTAT3500.08.4250391
GTATTAT8250.08.4168031
GTATCAA33400.08.3729621
GTCCAAA3950.08.1876651
GTATAGG4250.08.0573431
ACCCCCG1304.1894178E-48.0381885
GTCTAAG3450.07.99571231
Produced by FastQC (version 0.11.5)