FastQCFastQC Report
Wed 25 May 2016
SRR1294917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552252
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106490.2997816596345079No Hit
GGTATCAACGCAGAGTACTTTTTTT67090.18886610522001254No Hit
TATCAACGCAGAGTACTTTTTTTTT58310.1641493903022646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGT750.00264713310.1341976
TACTGCG951.6467985E-49.99902257
CCAAACG951.6467985E-49.99902259
GGTATCA27450.09.9736791
ACTGCGT800.00452150739.4990718
CAATGCG800.00452150739.4990719
ATAGGAC3250.09.0626943
CTTAGAC3250.09.0626943
TATACCG950.0018232469.0007675
TAGGACT5300.08.9630284
CGTACAC850.00743091158.9423163
TAGACGT850.00743348668.9419384
TACGTCT850.00743348668.9419384
GCGGGGT850.00744465238.94030215
TAGGACA4300.08.8379634
CTCGATC2151.0440999E-98.83634618
GTAGGAC2800.08.8225533
TAGGACC2800.08.822184
ATCCCGT1107.141971E-48.63716
TATACTG9050.08.6084685
Produced by FastQC (version 0.11.5)