FastQCFastQC Report
Wed 25 May 2016
SRR1294916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138575
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT500.001502615613.2972578
GAACCGT907.486E-611.6087176
TAGACCG800.00451724939.5002245
ACCGATC800.00452581939.4980418
GTCTTAG5350.09.4267151
GTATTAG7800.09.1496591
GTATAAG5750.09.1019231
TAGACAG6000.09.0252125
GGTATCA20350.09.0246671
GTGTAGG5600.08.8359571
GTCGTAG1000.0028635378.5640811
TAATACT6750.08.4457664
GCTCGTT1252.750201E-48.3585811
GTATCAA35100.08.3228021
CTAGACA4000.08.3138024
CTAGGAC4350.08.300153
GTTCTAA5350.08.1816771
TTAGACA5350.08.1695034
TTATACT5950.08.1441334
AGCACCG1752.4413985E-68.1430495
Produced by FastQC (version 0.11.5)