FastQCFastQC Report
Wed 25 May 2016
SRR1294916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138575
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72680.175616003092852No Hit
GGTATCAACGCAGAGTACTTTTTTT41660.10066266770567163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC500.001499822413.3005423
TACGCTG500.001500095613.300225
ACCGCTC600.005881436611.0825798
ATTAGAC4050.09.8522533
TGTACCG1356.4494634E-79.85201555
GGTATCA24000.09.5452771
TAACGCT800.00451751249.5001574
GTACCGT1106.840839E-59.5001566
TACCGTG1304.246742E-69.4993547
GTATCAA43550.09.4729291
GTATTGG4300.09.2846081
TGTGCGA1304.375803E-58.76863510
TAGGACT4450.08.7529544
GTATTAG6300.08.7512521
TAGACAG6050.08.6365065
TGAACCG1107.1474817E-48.6365065
GGGTACG1000.0028908598.5551021
TGGACTG5950.08.4623255
TTAGGAC3500.08.4146283
GTATTAA7550.08.3095921
Produced by FastQC (version 0.11.5)