FastQCFastQC Report
Wed 25 May 2016
SRR1294915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4326448
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC125120.2891979748745391No Hit
CCCATGTACTCTGCGTTGATACCAC117780.27223255659145795No Hit
GAGTACATGGGAAGCAGTGGTATCA54090.1250217268299538No Hit
GTATCAACGCAGAGTACTTTTTTTT43930.10153825956072972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGCG1106.066115E-610.36162957
GTCCTAA3550.09.6484551
GTCCGGA1106.7294466E-59.5144481
CATCCGG1502.7125807E-68.86474510
ACACCGT1201.7688127E-48.7066486
GTGTTAG6500.08.6361911
CTAGACT4100.08.5745864
CTAATAC5100.08.5700423
AGACCGT1451.6948215E-58.5155946
TCTGTCG3150.08.442818
CTTACAC5550.08.3887723
GTCTAGA5000.08.3727151
CCTAATA4800.08.3138712
GACTGTC4000.08.310897
TGCGTAT1150.00111363638.25907910
TAGGACC2658.913048E-118.2466444
CTGTCGC3350.08.2220989
GTATTAA8950.08.1856151
TAGGACG1401.0379062E-48.14424
ACCGTGG2105.8347723E-88.141288
Produced by FastQC (version 0.11.5)