FastQCFastQC Report
Wed 25 May 2016
SRR1294915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4326448
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC125850.29088527124329244No Hit
GTACATGGGAAGCAGTGGTATCAAC125450.28996072528781114No Hit
GTATCAACGCAGAGTACTTTTTTTT117690.2720245337514747No Hit
GGTATCAACGCAGAGTACTTTTTTT74720.17270518448389996No Hit
TATCAACGCAGAGTACTTTTTTTTT63750.14734951165482632No Hit
GAGTACATGGGAAGCAGTGGTATCA55650.12862745605633075No Hit
CATGTACTCTGCGTTGATACCACTG44570.10301753308949975No Hit
GCGTTGATACCACTGCTTCCCATGT43830.1013071230718594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG803.776087E-410.686749
GATCCGT750.002648736410.133556
GACCGGC750.002650768710.1326127
CCGATTT951.6465441E-49.99928914
TCCTACG1106.825123E-59.5021792
CGATTTT900.00111508469.49932415
CTTACAC6100.09.344573
CGCTATA850.0074252528.9432272
TGGACCG850.00743793468.9413665
AAAGCGA1201.7664678E-48.70771318
TTACACT7350.08.5308924
CTAATAC6250.08.51227953
GTATTAA8350.08.4241121
TCCGTAC1150.00111203278.26028258
TACACTG10100.08.1833425
AGCACCG1050.00451434878.143035
GTATAGA6450.08.1055381
GTGTTAG7450.08.03828051
TATACTG8350.07.96424155
GCACCGT1200.00169147977.91683536
Produced by FastQC (version 0.11.5)