FastQCFastQC Report
Wed 25 May 2016
SRR1294914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668856
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC103480.28204977246313295No Hit
CCCATGTACTCTGCGTTGATACCAC98170.26757659608335677No Hit
GAGTACATGGGAAGCAGTGGTATCA42800.11665761752437272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC700.001494654610.8550398
GCACCGT1355.7118086E-810.5535096
TAGACAG5400.010.2040925
TGGACCG800.00451650149.5003615
TGCACCG1002.7560786E-49.5003615
ACCGACC800.004525159.4981588
GTTCTAA4150.09.3969261
GGACCGA950.00182790988.9982566
GTCTAAG3500.08.9680071
GTCTTAC3400.08.9520211
GACATCG850.00745061928.9394437
CTAAGAC3300.08.9251913
ATACCGT1201.7686251E-48.7066456
GTATAGG4500.08.6660541
ATAGGAC3300.08.6372813
CTCGCTC2356.166374E-108.4902643
TCTCGCT2701.4551915E-118.4453422
CGCTCTG2701.4551915E-118.4447665
GTCTTAG3050.08.4200361
CCCCTAG2502.3101165E-108.370141
Produced by FastQC (version 0.11.5)