FastQCFastQC Report
Wed 25 May 2016
SRR1294914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668856
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC104590.2850752387120127No Hit
CCCATGTACTCTGCGTTGATACCAC104130.2838214418881526No Hit
GTATCAACGCAGAGTACTTTTTTTT91780.2501597228127787No Hit
GGTATCAACGCAGAGTACTTTTTTT55310.1507554398428284No Hit
TATCAACGCAGAGTACTTTTTTTTT50760.13835375386769064No Hit
GAGTACATGGGAAGCAGTGGTATCA44070.12011918701633426No Hit
CATGTACTCTGCGTTGATACCACTG37040.10095790077342912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC550.00307177812.0898778
GTGTACG750.002635968410.1393781
TGTACCG856.593585E-410.0594735
GGTCGTG800.00452110369.4991897
TATACAG7850.08.9559925
ATACCGT1107.1435823E-48.6369216
TCCGAGC2201.6098056E-98.63562713
GTCTTAC4000.08.55509951
GTCCTAG4150.08.4749321
GTCTAGG3600.08.4494811
CTAGACT3850.08.3901524
CCTAGAC4100.08.3421153
TATACTG8000.08.3130365
TAGACTG5150.08.3015075
GTCTTAG3100.08.2791281
TGGACAG5200.08.2216845
GTATTAA8950.08.178061
CTTACAC4700.08.0857393
GTATTAT8000.08.0798171
TAGACAG5300.08.0665595
Produced by FastQC (version 0.11.5)