FastQCFastQC Report
Wed 25 May 2016
SRR1294913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437766
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC91210.20553134167056128No Hit
CCCATGTACTCTGCGTTGATACCAC86370.1946249531859048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA750.002652959210.13161410
GACCGTG1502.6584894E-79.4983877
CTTATCG950.00182766638.9984729
TAGCGCT850.00743313658.942084
GTCTAGA3950.08.9120971
GTATTAG6350.08.8400271
AGACCGT1304.381252E-58.7677436
GTCTTAG4250.08.7307391
CAGCGTA1107.1609934E-48.6348989
GTCCTAA3650.08.6019381
GCGGACC1000.00291077238.54864513
CCTATAC4600.08.468153
CTAGGAC4600.08.468153
GTCTAGC2553.43789E-108.2083851
TAGGACC3250.08.1854414
TCAAGAC5000.08.17073253
CTACACT6550.08.1229574
GCACCGT2002.315046E-78.0736296
TAATACT8750.08.0350974
GGCGTCT1200.00169468717.91532238
Produced by FastQC (version 0.11.5)