FastQCFastQC Report
Wed 25 May 2016
SRR1294913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437766
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96360.21713627983088787No Hit
GTACATGGGAAGCAGTGGTATCAAC92790.20909169163042846No Hit
CCCATGTACTCTGCGTTGATACCAC92640.20875368372284617No Hit
GGTATCAACGCAGAGTACTTTTTTT55520.12510799352647256No Hit
TATCAACGCAGAGTACTTTTTTTTT53980.12163777900862731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC802.871399E-511.87409910
GCGTTAT856.566527E-410.064071
CGTACAC1054.105182E-59.9531973
AACCGTG900.00111514529.4992787
TGAACCG1151.11067064E-49.0875985
TGCGCAC850.0074439258.94049810
CTAGCAC4000.08.7882193
TACACCG1304.3689157E-58.769855
GCGTGCG1201.7665852E-48.7076729
CTGTGCG2201.6098056E-98.6357099
CTAGGAC4850.08.6192633
TATACAG10000.08.4555975
GTCCCGC1252.729869E-48.3643611
TGTCGCC2752.3646862E-118.29027910
CAGGACT7000.08.2791564
GTACCGT1150.00111048778.2614536
CCAGGAC9550.08.1577353
GTCTTAG4200.08.1471041
ACACCGT2355.522452E-98.0856766
GTATTAG6750.08.0264061
Produced by FastQC (version 0.11.5)