FastQCFastQC Report
Wed 25 May 2016
SRR1294912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834661
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC68330.17819045803527353No Hit
CCCATGTACTCTGCGTTGATACCAC63310.16509933994165324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA655.464231E-513.15187610
TACACCG1104.9761E-711.22705655
ACACCGT951.364182E-510.9980666
TAAACCG750.002649508910.1331395
GTATAGG5400.09.866731
CTAGACT3400.09.7810114
TAGGACC2650.09.6808294
GTTCTAG5650.09.2617531
TTAGACA4850.09.0117824
CAGACGT950.00182201559.00147154
AATATCG850.00744007958.9410055
GGACAGT4850.08.8128836
TAGGACT5050.08.6548814
ACTGTCG1107.160866E-48.6348468
TCTGTCG2554.0017767E-118.5671218
TTACACC5400.08.4458264
CTAGCAC3400.08.3833963
CTTACAC7100.08.2968063
TACACTG8150.08.2759145
GCTGCGC1150.00111311368.2594178
Produced by FastQC (version 0.11.5)