FastQCFastQC Report
Wed 25 May 2016
SRR1294912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834661
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89910.23446661908314714No Hit
CCCATGTACTCTGCGTTGATACCAC68440.17847731520465565No Hit
GTACATGGGAAGCAGTGGTATCAAC67090.17495679539860237No Hit
GGTATCAACGCAGAGTACTTTTTTT55000.14342858469105874No Hit
TATCAACGCAGAGTACTTTTTTTTT48250.12582598566079245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1103.8187864E-812.0911496
TAACGCT1001.9313575E-611.4002274
TACACCG1451.445369E-810.4829675
ATAACGC1106.04822E-610.3641143
GTCCTAC2500.09.5048991
TGGACCG800.00451725869.5001895
TACGTCC900.00111409569.5001894
CGTTCCG900.00111489319.49944610
CCGTTCA1002.7588214E-49.4994469
AGGACCG1252.7556598E-59.1201825
TCGTGCA850.00744246838.9406569
TAGGACC2451.6370905E-118.9185454
CTAATAC5200.08.7696343
ACCGAGC1201.7660837E-48.7078258
GTTCTAG4700.08.69597151
TCTATAC5250.08.6861143
CTAGTAG3200.08.6097713
AGGACTG6850.08.5987125
GTAGGAC3000.08.5503933
GACATCG1000.00290794958.5495017
Produced by FastQC (version 0.11.5)