FastQCFastQC Report
Wed 25 May 2016
SRR1294911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209097
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26910.12181447894773294No Hit
GTACATGGGAAGCAGTGGTATCAAC26550.12018485381130842No Hit
CCCATGTACTCTGCGTTGATACCAC24600.11135771765567559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG2100.010.4203461
GTCCTAG1659.977157E-99.802541
GACCCGA900.00111575229.49827811
GCACCGT1106.853453E-59.4980636
CTAATAC3350.09.0764783
ATTACGT950.00182070779.0018363
GTATAAG3400.08.9545691
CTAGACA2553.6379788E-128.9432034
ATAGGAC1701.6799459E-78.9430013
CTAAGAC2252.6011548E-108.8684753
GACAGTG3550.08.8291857
CTTACTC3250.08.771023
GTTCTAG3050.08.7343741
TTAGACT2304.0199666E-108.67587954
TGGACTC2751.8189894E-128.6373425
TCCTATA3200.08.6111322
GTTACAC1554.1959593E-68.5823963
TGTCGCC1554.216521E-68.57928510
GTATTAA4000.08.5628071
GTCTCGC1000.00286630638.5628071
Produced by FastQC (version 0.11.5)