FastQCFastQC Report
Wed 25 May 2016
SRR1294911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209097
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69660.3153324638981448No Hit
GGTATCAACGCAGAGTACTTTTTTT44040.19935747502259973No Hit
TATCAACGCAGAGTACTTTTTTTTT39950.18084312277822115No Hit
CCCATGTACTCTGCGTTGATACCAC26650.12063752746031522No Hit
GTACATGGGAAGCAGTGGTATCAAC25740.11651819725435324No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22510.10189683839143325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCG803.7734827E-410.68705617
ATAAGAC2400.010.291473
GTGTAGG2450.010.086921
TAAGACA3350.09.9249594
GTCCTAT1651.0137228E-89.7930131
CCTATAC2106.730261E-119.4998193
CTAGACC1201.7017079E-59.4996034
GGTATCA19100.09.4054541
TTAGACT2650.09.3203664
TAGGACA2550.09.3133374
TGCGTTG8400.09.27342312
ATAGGAC1951.6825652E-99.2562343
TCTATAC2800.09.160543
TAGACCC1356.8523877E-69.1477665
GTAGGAC1054.4942138E-49.0474463
GCCCTAT850.0074049138.9458671
GTGTAGA3650.08.8539561
CTGCGTT8900.08.75244411
CCCTTAC1752.6045745E-78.6902691
ATAGCCT2659.094947E-128.6034154
Produced by FastQC (version 0.11.5)