FastQCFastQC Report
Wed 25 May 2016
SRR1294910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551291
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC114160.3214605618069598No Hit
CCCATGTACTCTGCGTTGATACCAC99110.27908160722396447No Hit
GAGTACATGGGAAGCAGTGGTATCA50540.1423144428321982No Hit
GTATCAACGCAGAGTACTTTTTTTT45740.1287982314037346No Hit
CATGTACTCTGCGTTGATACCACTG36960.10407482799916988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT3450.09.6534641
GTATACG1002.712137E-49.5155571
CGTAGAC900.00111210269.5020083
ACCGACT900.00111655549.4978588
TAGACAG5400.09.323685
TCTGTCG2151.07320375E-109.2769788
CCTATAC3600.09.2380643
TAATACT6700.09.0765464
TTAGACT3700.08.9883864
ATAGGAC4000.08.7893583
TGCACCG1107.1510294E-48.6359995
TCTGCGC1000.00291224248.5480728
CTTGGAC3700.08.4747653
TCTAGAC3600.08.446233
GTATTAG6250.08.2214411
TAGGACT5100.08.1978114
CCAGTAC3600.08.1822853
AATACTG6750.08.1626195
GTCTAAT3850.08.1561921
TCTACAC4450.08.1140763
Produced by FastQC (version 0.11.5)