FastQCFastQC Report
Wed 25 May 2016
SRR1294910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551291
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115690.3257688541997825No Hit
GTACATGGGAAGCAGTGGTATCAAC111590.3142237569379699No Hit
CCCATGTACTCTGCGTTGATACCAC107460.3025941833547293No Hit
GGTATCAACGCAGAGTACTTTTTTT76130.2143727450101949No Hit
TATCAACGCAGAGTACTTTTTTTTT65070.18322914117710998No Hit
GAGTACATGGGAAGCAGTGGTATCA51150.14403212803456544No Hit
CATGTACTCTGCGTTGATACCACTG39800.11207191976101084No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37850.10658095886819752No Hit
GCGTTGATACCACTGCTTCCCATGT36750.10348349374917461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC508.724093E-515.1989538
CGTGCGG855.330836E-511.175710
TCTAGAC3700.09.5004163
CCTATAC3350.09.3586183
CGTGCTG950.0018258048.9993810
TACGGAA1451.6864691E-58.5188132
TTATACT5800.08.5174944
CTATACT5250.08.5050134
GTATAGA5850.08.4493351
CGGAACG1356.8078996E-58.4438639
CCTACAC3950.08.418093
CTAGACT4750.08.4002494
GGACTGC3400.08.3826026
TAGACAG6350.08.37820155
CTAGGAC3300.08.348853
CTAGTAC2409.331416E-108.3128633
TGTACTG5600.08.3127465
TCTATAC4250.08.2709493
CTGCTCG1150.00111188638.26039
GTGCGGC1150.00111188638.260311
Produced by FastQC (version 0.11.5)