FastQCFastQC Report
Wed 25 May 2016
SRR1294909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516577
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC56030.15933107678290565No Hit
CCCATGTACTCTGCGTTGATACCAC51230.1456814396499778No Hit
GTATCAACGCAGAGTACTTTTTTTT43950.12497948999837057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGT400.00527665714.2517094
AGCCGCG2100.011.7633152
CACCGTA700.001495153610.8545817
GCCGCGG2050.010.6598143
CGCTACA750.002646359410.1345482
TGCACCG750.002650112110.1328175
AGCCTCG3450.09.6388372
CCCGATC800.0045266439.4977598
GTATTAG5350.08.8927361
GCGCTGA1401.0891896E-58.8193476
ATCGTCC1107.164779E-48.6343268
GTCTAGG3650.08.6028091
TACGACC1000.00290313748.5510254
TAATACC5850.08.44545654
GTCCAGA4750.08.4134641
CGATTTC1150.00111105488.26091916
TATACTG6900.08.2604485
TGGACTG6100.08.2536785
AGGACCG1502.5894351E-58.2329145
GGACCAT2201.4719262E-88.202616
Produced by FastQC (version 0.11.5)