FastQCFastQC Report
Wed 25 May 2016
SRR1294909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516577
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110280.3136004131290172No Hit
GGTATCAACGCAGAGTACTTTTTTT70580.20070653934209318No Hit
TATCAACGCAGAGTACTTTTTTTTT64210.18259233339693687No Hit
GTACATGGGAAGCAGTGGTATCAAC56640.16106571816854856No Hit
CCCATGTACTCTGCGTTGATACCAC54100.15384278518570757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG400.00527915214.2506945
AATACCG658.024566E-411.6928775
ACAACGC855.331369E-511.1755838
ATCGTCG700.001493201110.8562829
TACGAGT750.00264792410.1338264
TTGCGTG951.6464813E-49.99920716
AGACCGT1151.0267273E-59.9135266
CTCGTTC1151.02829E-59.91225712
ACCGCGC800.0045207999.49924718
GCGTCGC900.00111505889.49924714
GTCTCGC1856.6320354E-99.2492171
GTATTAG4550.09.1927511
GTATAGG3000.09.1892691
TGGACAG4900.09.1126885
GTGGTCG1151.1123103E-49.0862369
AGGACTG6500.09.0619795
TAACACG950.00182384359.0004384
TTACACG950.00182384359.0004384
TAGACTG5150.08.85485
TCTATAC4250.08.7181953
Produced by FastQC (version 0.11.5)