FastQCFastQC Report
Wed 25 May 2016
SRR1294908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161341
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC101150.2430706832244702No Hit
CCCATGTACTCTGCGTTGATACCAC90410.2172616952083475No Hit
GTATCAACGCAGAGTACTTTTTTTT51970.12488762636851919No Hit
GAGTACATGGGAAGCAGTGGTATCA43260.10395687351745506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG600.005877107411.0836775
GCACCGT856.608422E-410.0570796
AGCGCAA1054.1154566E-59.95089310
GACTGCG800.00452460359.4983537
GTCTTGG5250.09.2413061
ACACCGC1151.1135806E-49.0853816
GTGTAGA6300.09.0601041
CTGTACG1054.489764E-49.048774
CCGTGTA850.0074497198.9396269
CTGTTCG850.0074497198.9396269
GTATTAG8100.08.925881
GTCTAGG3500.08.69770051
TAGACTG6850.08.5988065
GTCCTAC2553.8198777E-118.5804521
TCTGTCG2803.6379788E-128.4806728
GTGTTAG5550.08.3989621
GTCCTAT3900.08.2934811
GACGTGG1150.00111313648.2594376
TAGGACT5300.08.2463324
CTATACT7150.08.2388114
Produced by FastQC (version 0.11.5)