FastQCFastQC Report
Wed 25 May 2016
SRR1294908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161341
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT123300.2962987171683359No Hit
GTACATGGGAAGCAGTGGTATCAAC98550.23682269729877942No Hit
CCCATGTACTCTGCGTTGATACCAC96820.23266538358668515No Hit
GGTATCAACGCAGAGTACTTTTTTT81300.19536971375333098No Hit
TATCAACGCAGAGTACTTTTTTTTT71200.17109869150353216No Hit
GAGTACATGGGAAGCAGTGGTATCA43950.105614992859273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGT250.006036475318.9985110
AGAACCG855.3246913E-511.1770715
TATACCG700.001491644310.8577265
GACCGTA803.7762756E-410.6866627
TAGTACG1002.3996039E-510.4505624
CGTTCAC800.00452106079.49925510
AGGACGG1252.7547369E-59.1204915
GCGAGAA950.00182609988.99929413
CTAGGAC4650.08.9900553
CCGTGTA850.0074439218.9404759
CCAGTAC4850.08.8152253
CTAATAC5950.08.7823023
TGTCGCC3400.08.66108510
GGCGTGC1451.6923117E-58.5165748
CTGTCGC3800.08.4993339
CCGTTCA1356.809526E-58.4437829
TCTGTCG3500.08.4136268
TCTATAC5450.08.3677243
GTCTTAG5150.08.3058091
GTAGAAC3450.08.2616123
Produced by FastQC (version 0.11.5)