FastQCFastQC Report
Wed 25 May 2016
SRR1294907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554610
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC49160.13829927896449962No Hit
GTATCAACGCAGAGTACTTTTTTTT46460.1307035089644152No Hit
CCCATGTACTCTGCGTTGATACCAC45030.12668056411251868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG856.595997E-410.0590545
GCCCTAC1304.1717594E-69.5125441
GTATAGG5200.09.329611
CTGTCGC2651.8189894E-128.96007259
TATTCCG850.0074372848.9413815
TGTCGCC2607.2759576E-128.7672110
GCACGCC1201.7690154E-48.70644911
GTCCTAG3900.08.5368991
GTCTCGC1803.9575207E-78.4555941
TCGTGGG1804.0511623E-78.44261712
TCTGTCG2701.4551915E-118.4426178
TAGCACC2303.6889105E-98.2621074
CTAGGAC3800.08.251123
GTCTAGA4850.08.2376661
ATAGGAC3250.08.1857263
CCCCTAC2803.274181E-118.1536081
GTATAAG5950.08.1536081
TTAAGCT3500.08.1440764
GTATAAA9250.08.1242271
GTACTAT4700.08.0957821
Produced by FastQC (version 0.11.5)