FastQCFastQC Report
Wed 25 May 2016
SRR1294907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554610
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114510.32214504544802386No Hit
GGTATCAACGCAGAGTACTTTTTTT71860.20216001192817212No Hit
TATCAACGCAGAGTACTTTTTTTTT64600.18173583037238966No Hit
GTACATGGGAAGCAGTGGTATCAAC49440.13908698844598985No Hit
CCCATGTACTCTGCGTTGATACCAC49260.13858060377931755No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35800.10071428370482276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG752.073736E-411.4008944
AGACCGT700.001491244110.8579956
GACCGCT700.001493345510.85616211
GTACGGA803.7538802E-410.6934541
CCTACGC750.00265099210.13241813
TAAACCG951.6438727E-410.0007855
CTAGACT3950.09.1399574
GTCTAGA3500.08.9621321
GTCTAGG3700.08.7345931
TATACTG7950.08.7239555
TCTGTCG2401.0186341E-108.7075468
TAGACTG5250.08.6863965
TAGACAG6150.08.6510865
GTCTAAG3300.08.6411741
GTGTAGG3850.08.6411741
CTGTCGC2252.4556357E-98.4436829
TTAGTAC3050.08.4106153
GTCTCGC1701.6002305E-68.3870221
CTAAGAC4200.08.36982253
CTTACAC5450.08.3677463
Produced by FastQC (version 0.11.5)