FastQCFastQC Report
Wed 25 May 2016
SRR1294906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589266
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52800.20391879397481755No Hit
CCCATGTACTCTGCGTTGATACCAC49120.18970627197051212No Hit
GTATCAACGCAGAGTACTTTTTTTT38540.1488452712081339No Hit
GAGTACATGGGAAGCAGTGGTATCA26360.10180491305257938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC400.00528663514.2474499
AGGACCG707.2659404E-613.5716225
CGTACCA604.09918E-412.66464310
GAACCGT550.003073067412.0889786
CCGTACT752.0719512E-411.4017054
CAAGACG752.0719512E-411.4017054
TTCCGTA752.0779113E-411.3981797
GTCTCGC1158.68642E-710.7521691
TATTCCG1002.3996869E-510.4501495
TAGACTG3900.09.9873225
GTCCTAG2600.09.5115351
GTCTTAG2700.09.5115351
CCGTTCA1002.7613877E-49.4982999
ATTATAC4850.09.4032863
TAAGACT3550.09.3675994
GTAGGAC1651.0641452E-79.2133213
TAATACT5050.09.0310534
GGACCGC850.0074472998.9397486
ACACGGT1401.08773675E-58.820026
GTGTAGG2701.8189894E-128.8069761
Produced by FastQC (version 0.11.5)