FastQCFastQC Report
Wed 25 May 2016
SRR1294906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589266
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94270.36408001340920554No Hit
GGTATCAACGCAGAGTACTTTTTTT63220.24416185899787815No Hit
GTACATGGGAAGCAGTGGTATCAAC54250.20951883661238357No Hit
TATCAACGCAGAGTACTTTTTTTTT51600.19928427592993536No Hit
CCCATGTACTCTGCGTTGATACCAC50950.1967739119889575No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32350.12493888229328312No Hit
GAGTACATGGGAAGCAGTGGTATCA27550.10640081011375425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG751.4806903E-512.6656627
CGTCAGA1158.823463E-710.7391095
CTGCGCT803.7752677E-410.6866539
TAGGACC1909.094947E-1210.4999794
GTCCTAT2950.010.3112361
CAGCGTC1201.5202131E-610.2940322
GCGTCAG1201.5247406E-610.2916464
CTGTCGC1409.707401E-810.1777659
TAATACT4850.09.7937954
AGCGTCA1304.238822E-69.5003473
GTCTTAA2451.8189894E-129.3116781
TCTATAC3850.09.1302053
ACACCGT850.0074376728.9411586
ATAGGAC2356.548362E-118.8939433
GCTAGAC1401.08504355E-58.8217523
GTAACAC2502.5465852E-118.740323
GTATAGG3050.08.7265181
GTACTAG1752.6026282E-78.69091
GTCCTGG4500.08.6607231
TTAGCAC1651.0498734E-68.636683
Produced by FastQC (version 0.11.5)