FastQCFastQC Report
Wed 25 May 2016
SRR1294905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404352
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC78860.2316446712913353No Hit
CCCATGTACTCTGCGTTGATACCAC71120.20890906698249767No Hit
GTATCAACGCAGAGTACTTTTTTTT45000.13218374598161414No Hit
GAGTACATGGGAAGCAGTGGTATCA35590.10454265598856993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5450.09.7734431
CTAGTAC1804.1763997E-99.5014493
GTGCTAG3750.09.1311891
GTAGGAC2850.09.0013723
CGTTACT950.00182623818.99912315
AACCCGT850.00744898858.9396486
GTCTAAC1401.0692154E-58.8322521
GTATAGG3800.08.7607351
CTGAACG1201.7683674E-48.70671759
TCTATAC3750.08.6146463
ACTGTCC5300.08.6023038
ACCGACC1000.00291074258.5485398
AGGACTG6800.08.522235
GCTAGAC1451.6870765E-58.5185393
GGACTGT4800.08.5089636
TAGGACC2803.6379788E-128.4834364
GTCTAAG2252.395609E-98.4548041
TAGACTG3400.08.3825225
ACGCTGA1252.749857E-48.3585716
GGTTAGA2303.614332E-98.2710051
Produced by FastQC (version 0.11.5)