FastQCFastQC Report
Wed 25 May 2016
SRR1294905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404352
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT119430.3508156618352039No Hit
GTACATGGGAAGCAGTGGTATCAAC79790.2343764687082887No Hit
CCCATGTACTCTGCGTTGATACCAC76500.22471236816874401No Hit
GGTATCAACGCAGAGTACTTTTTTT75400.2214812099336379No Hit
TATCAACGCAGAGTACTTTTTTTTT68360.2008017972289587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35660.10474827514898577No Hit
GAGTACATGGGAAGCAGTGGTATCA35650.10471890098321208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGT856.5972394E-410.0588246
CCGTCCC856.6008035E-410.0582329
GTCTAGA3200.09.8022021
CTAGACA3000.09.5001394
TAACACG900.0011140819.5001394
TCGGCGC800.0045199729.49944214
CCAGGAC7350.09.04801753
ATACCGT1054.494945E-49.047626
TAGGACC1901.0588337E-89.0001324
CGCATGA850.00744213778.94065118
CCCTTAA2451.6370905E-118.9232171
CCTAGAC3650.08.5894193
CAGGACA7200.08.4445694
TCCTATA3500.08.4156462
TAGTACA4750.08.4001244
ATTGGAC2502.3646862E-108.3603693
TAAGACT4600.08.260994
AACCGTG1150.00111174518.2603847
ATAGTAC3600.08.1809163
TACACTG7900.08.1772165
Produced by FastQC (version 0.11.5)