FastQCFastQC Report
Wed 25 May 2016
SRR1294904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980622
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC85280.21423787538731384No Hit
CCCATGTACTCTGCGTTGATACCAC76780.1928844286144226No Hit
GTATCAACGCAGAGTACTTTTTTTT54030.13573255636933124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT658.041499E-411.6900096
GTCTAGG3250.09.9525231
CTAGGAC2900.09.5009993
TGTACCG1304.2433458E-69.4999245
ACCGCTC900.00111632199.4981348
TAGGACA3750.09.374324
TAGGACT5000.09.120964
GTATTAG6050.09.1203231
TCTACGC1054.4906407E-49.0485713
TGTGCGA1054.504205E-49.04584210
TAGACAG5900.09.0168775
TACGCTG950.00182489148.9999295
GTCTTGG6150.08.6626451
GTCGTAC1000.00286950058.5620971
GTCTTAA5150.08.4974431
CTGTCCA7000.08.4125279
AGGACCG1252.745326E-48.3599345
AAAGCCG1252.745326E-48.3599345
TGCACTG8000.08.3124345
CTGAACG1502.594884E-58.2316119
Produced by FastQC (version 0.11.5)