FastQCFastQC Report
Wed 25 May 2016
SRR1294904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980622
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133550.33550033135525054No Hit
CCCATGTACTCTGCGTTGATACCAC83950.2108966890099085No Hit
GTACATGGGAAGCAGTGGTATCAAC83730.2103440115640219No Hit
GGTATCAACGCAGAGTACTTTTTTT81180.20393797753215454No Hit
TATCAACGCAGAGTACTTTTTTTTT77430.1945173392499966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC604.0968508E-412.66581257
CCGTGCG600.005881341611.0825869
CTTAGAC1951.6370905E-109.7440353
TAGAACA6400.09.351994
GTATAGG4650.09.1983591
GTCGTAG950.00181609539.0047091
TCTAACG850.00742857348.9427082
TAACGCT850.0074362328.9415844
GTTCTAT4050.08.6835541
GTATTAG5850.08.4488631
GTATTAA7150.08.375011
GTATTGG4900.08.3410971
GTAAGAC3550.08.2961533
GTATTAT6600.08.2088381
GTTCTAA3950.08.1814941
TCTACAC4900.08.14322953
TATACTG8150.08.0433125
TTATACT7800.08.0388284
CTAGGTC2502.073648E-97.9803643
GTATCAA73050.07.9240621
Produced by FastQC (version 0.11.5)