FastQCFastQC Report
Wed 25 May 2016
SRR1294903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298375
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC183646.154671135316296No Hit
CCCATGTACTCTGCGTTGATACCAC179556.0175953079178885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA141374.737997486384583No Hit
GAGTACATGGGAAGCAGTGGTATCA87442.9305404273146207No Hit
CATGTACTCTGCGTTGATACCACTG71492.395978215333054No Hit
GCGTTGATACCACTGCTTCCCATGT59001.977377461248429No Hit
ACGCAGAGTACATGGGAAGCAGTGG56371.8892333472978633No Hit
GGTATCAACGCAGAGTACATGGGAA53341.787683284457478No Hit
TATCAACGCAGAGTACATGGGAAGC52041.7441139505655634No Hit
GTATCAACGCAGAGTACATGGGAAG50111.6794302471721825No Hit
ACTCTGCGTTGATACCACTGCTTCC46621.5624633431085044No Hit
GCTTCCCATGTACTCTGCGTTGATA34781.1656472559698368No Hit
GTGGTATCAACGCAGAGTACATGGG33921.136824465856724No Hit
GTACTCTGCGTTGATACCACTGCTT31601.0590699622957687No Hit
ATACCACTGCTTCCCATGTACTCTG27300.9149560117302052No Hit
GATACCACTGCTTCCCATGTACTCT27250.9132802681189779No Hit
GGGAAGCAGTGGTATCAACGCAGAG25430.8522832006702974No Hit
CAGTGGTATCAACGCAGAGTACATG24600.8244658567239213No Hit
CTGCTTCCCATGTACTCTGCGTTGA23540.7889400921658987No Hit
CATGGGAAGCAGTGGTATCAACGCA22480.753414327607876No Hit
GCAGAGTACATGGGAAGCAGTGGTA21620.7245915374947633No Hit
TACCACTGCTTCCCATGTACTCTGC21520.7212400502723083No Hit
GTTGATACCACTGCTTCCCATGTAC20980.7031420192710515No Hit
GCAGTGGTATCAACGCAGAGTACAT20950.702136573104315No Hit
ACATGGGAAGCAGTGGTATCAACGC20330.6813573523250942No Hit
CCACTGCTTCCCATGTACTCTGCGT18770.6290741516547969No Hit
CTTCCCATGTACTCTGCGTTGATAC18680.6260578131545873No Hit
AAGCAGTGGTATCAACGCAGAGTAC16380.5489736070381231No Hit
CCATGTACTCTGCGTTGATACCACT16150.5412651864264767No Hit
ATGGGAAGCAGTGGTATCAACGCAG16090.5392542940930038No Hit
GTATCAACGCAGAGTACTTTTTTTT14730.49367406786761625No Hit
TTCCCATGTACTCTGCGTTGATACC14100.47255969836614997No Hit
TGATACCACTGCTTCCCATGTACTC13310.44608294930875575No Hit
ATCAACGCAGAGTACATGGGAAGCA12260.4108923334729786No Hit
GGTATCAACGCAGAGTACTTTTTTT12010.40251361541684116No Hit
GTACATGGTAAGCAGTGGTATCAAC11490.3850858818600754No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA11210.3757017176372015No Hit
TATCAACGCAGAGTACTTTTTTTTT9080.3043150397989107No Hit
GGAAGCAGTGGTATCAACGCAGAGT8530.28588186007540844No Hit
ACCATGTACTCTGCGTTGATACCAC8390.2811897779639715No Hit
CTCTGCGTTGATACCACTGCTTCCC6630.22220360284876411No Hit
AAAAAGTACTCTGCGTTGATACCAC6530.21885211562630916No Hit
TCAACGCAGAGTACATGGGAAGCAG6470.21684122329283623No Hit
CTGCGTTGATACCACTGCTTCCCAT6330.21214914118139921No Hit
TGGGAAGCAGTGGTATCAACGCAGA6150.2061164641809803No Hit
AAACAAAAAAAAAAAAAAAAAAAAA5700.19103477167993296No Hit
TCCATGTACTCTGCGTTGATACCAC5690.19069962295768747No Hit
TCCCATGTACTCTGCGTTGATACCA5350.1793045664013406No Hit
CCCCATGTACTCTGCGTTGATACCA5070.16992040217846668No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5010.16790950984499373No Hit
GTACATGGAAAGCAGTGGTATCAAC4820.16154168412232928No Hit
AAAGTACTCTGCGTTGATACCACTG4770.15986594051110178No Hit
ATGTACTCTGCGTTGATACCACTGC4760.15953079178885632No Hit
AGTGGTATCAACGCAGAGTACATGG4720.15819019689987432No Hit
GGTAAGCAGTGGTATCAACGCAGAG4680.15684960201089235No Hit
AACGCAGAGTACATGGGAAGCAGTG4640.15550900712191035No Hit
GAGTACATGGTAAGCAGTGGTATCA4290.14377880184331795No Hit
TTGATACCACTGCTTCCCATGTACT4180.1400921658986175No Hit
ACTGCTTCCCATGTACTCTGCGTTG4150.13908671973188103No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4120.13808127356514452No Hit
GCTTACCATGTACTCTGCGTTGATA4070.13640552995391705No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA3730.12501047339757018No Hit
GTACATGGGTAAGCAGTGGTATCAA3700.12400502723083368No Hit
CAACGCAGAGTACATGGGAAGCAGT3530.11830749895266025No Hit
GAACAAAAAAAAAAAAAAAAAAAAA3480.11663175534143276No Hit
GAGTACATGGAAAGCAGTGGTATCA3450.11562630917469627No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3380.1132802681189778No Hit
AAAAACAAAAAAAAAAAAAAAAAAA3190.10691244239631335No Hit
GCGTTGATACCACTGCTTACCATGT3040.1018852115626309No Hit
CGTTGATACCACTGCTTCCCATGTA3020.10121491411813992No Hit
CGCAGAGTACATGGGAAGCAGTGGT3010.10087976539589444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA453.5124867E-516.8950062
GTACAAA551.1112645E-515.5615351
GTAAAAA653.3142987E-614.6305031
ACATGAA500.001490516713.3048183
GAACAAA2550.012.67976951
ACGCAAA600.00581186711.0947981
TACAAAA1252.201923E-710.6438542
GAGAACA909.3564086E-510.5664751
GAAAACA1553.5361154E-910.4301341
GGAAAAA1304.2777036E-58.7783011
CAGAAAA1304.3116048E-58.7724084
TGGTAAG3350.08.2227186
CATGGTA3500.08.1458074
ATGGTAA3500.07.87031655
AAAGTAC1951.3150966E-67.8029351
AAGTACT1951.3285025E-67.79769562
AGTACTC1951.3285025E-67.79769563
GAAAAAA7600.07.63288741
AAAAGTA1409.14764E-47.46698952
CCATGTA51700.07.46305042
Produced by FastQC (version 0.11.5)