##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294903_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	298375
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.942465018852115	31.0	30.0	34.0	23.0	34.0
2	30.040576455802263	31.0	30.0	34.0	23.0	34.0
3	30.44832509426058	31.0	31.0	34.0	25.0	34.0
4	33.49782320904902	37.0	35.0	37.0	26.0	37.0
5	34.32662253875157	37.0	35.0	37.0	30.0	37.0
6	34.07830749895266	37.0	35.0	37.0	28.0	37.0
7	34.28861667364893	37.0	35.0	37.0	30.0	37.0
8	34.29828236279849	37.0	35.0	37.0	28.0	37.0
9	35.59389694176791	39.0	37.0	39.0	27.0	39.0
10	35.78490825303729	39.0	37.0	39.0	29.0	39.0
11	35.799001256807706	39.0	37.0	39.0	29.0	39.0
12	35.969152911604525	39.0	37.0	39.0	30.0	39.0
13	35.58086635944701	39.0	37.0	39.0	27.0	39.0
14	36.86469040636783	40.0	37.0	41.0	28.0	41.0
15	36.781154587348134	40.0	37.0	41.0	27.0	41.0
16	36.63154754922497	40.0	37.0	41.0	27.0	41.0
17	36.73218600754085	40.0	37.0	41.0	27.0	41.0
18	36.57490071219104	40.0	37.0	41.0	27.0	41.0
19	36.45940846250524	40.0	36.0	41.0	27.0	41.0
20	36.52560703812317	40.0	36.0	41.0	27.0	41.0
21	36.40753079178886	40.0	36.0	41.0	27.0	41.0
22	36.51356514453288	40.0	36.0	41.0	27.0	41.0
23	36.49978718056138	40.0	36.0	41.0	26.0	41.0
24	36.60446753246753	40.0	36.0	41.0	27.0	41.0
25	35.67433263510683	40.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.6246076074937825
1101	2	-0.620000951584661
1101	3	-0.5031372115455284
1101	4	-0.6583824534572216
1101	5	-0.46895872104702363
1101	6	-0.28119457929674496
1101	7	-0.14096320363565695
1101	8	-0.43156279865095115
1101	9	-0.33584824443189376
1101	10	-0.2723377051013358
1101	11	-0.3997922800759568
1101	12	-0.3207634021415089
1101	13	-0.18596288465740685
1101	14	-0.4751057539894319
1101	15	-0.23642330481818163
1101	16	-0.20316332519909963
1101	17	-0.0816352025263214
1101	18	-0.36448916986924473
1101	19	-0.5225882646579691
1101	20	-0.46697359183628606
1101	21	-0.7637359875942593
1101	22	-0.9135340591090966
1101	23	-1.0962549974772955
1101	24	-0.9967258925050118
1101	25	-1.0916690745612243
1102	1	-0.6906466613459727
1102	2	-0.7289788239342343
1102	3	-0.3075347999647846
1102	4	-0.6852494403783709
1102	5	-0.37202800563289884
1102	6	-0.4192747718793086
1102	7	-0.5997653658907609
1102	8	-0.023748804174800853
1102	9	-0.4945730297646733
1102	10	0.2515846600532612
1102	11	-0.0778290556076513
1102	12	-0.034893287903081216
1102	13	-0.04346575942500408
1102	14	0.04959038038849428
1102	15	-0.1677512862477215
1102	16	-0.19038538621940404
1102	17	-0.1935093360659934
1102	18	-0.19082096641092505
1102	19	-0.15814296046994514
1102	20	-0.2861372762674037
1102	21	-0.42074665916146614
1102	22	-0.9731519030871283
1102	23	-0.3740542688446098
1102	24	-0.48213711860682196
1102	25	-0.7197332544195802
1103	1	-0.4162702441124111
1103	2	-0.3208155586281123
1103	3	-0.11479103579244665
1103	4	-0.6739115718976478
1103	5	-0.4876742641362952
1103	6	-0.1993201233532318
1103	7	-0.13037761078872023
1103	8	-0.19154925768953746
1103	9	-0.24740749688485408
1103	10	-0.42188472770184404
1103	11	-0.21615105107250798
1103	12	-0.07570961443369129
1103	13	-0.09335238074019969
1103	14	-0.4492758327634334
1103	15	-0.17908915472844456
1103	16	-0.015782211616226505
1103	17	0.12848612878661925
1103	18	-0.20669398228394442
1103	19	-0.49147629380327373
1103	20	-0.3314887501903101
1103	21	0.039570801155996094
1103	22	-0.08653058789438717
1103	23	-0.31963250013712496
1103	24	-0.2735203385614753
1103	25	0.11019871836953854
1104	1	0.5165878306243314
1104	2	0.09281473367145665
1104	3	0.3782819925056309
1104	4	0.2860331365793485
1104	5	-0.13947745116453092
1104	6	0.1654175525390471
1104	7	0.09920646028263036
1104	8	0.06970318849441526
1104	9	0.5609359318108247
1104	10	0.8249701786442571
1104	11	0.5596853108695328
1104	12	0.34579506049563946
1104	13	0.32310461301620563
1104	14	0.4531874356489496
1104	15	0.4667189401990228
1104	16	0.2491511589922908
1104	17	0.4083451043403059
1104	18	0.4717764926640271
1104	19	0.6408972035408667
1104	20	0.38029642049897205
1104	21	0.3419036064242249
1104	22	0.3550716237665341
1104	23	0.2640968820266991
1104	24	0.5280203690457697
1104	25	0.47206309882491126
1105	1	-0.3177745077897143
1105	2	-0.36107297614911005
1105	3	-0.35697429407228753
1105	4	-0.09579206118256423
1105	5	-0.5075075495631438
1105	6	-0.277311883437541
1105	7	-0.15904165856745323
1105	8	0.04822270743454027
1105	9	0.0020575473488051443
1105	10	-0.20917992747189373
1105	11	-0.037555669257805846
1105	12	-0.31257525270778075
1105	13	-0.6613316851380162
1105	14	-0.6361430679204076
1105	15	-0.25580340850507355
1105	16	-0.16319047069450932
1105	17	-0.08538439594909875
1105	18	-0.012209615767382331
1105	19	0.020021262965912
1105	20	-0.14179503232757895
1105	21	0.4341924996006341
1105	22	0.12264510116057892
1105	23	0.13428145981305306
1105	24	-0.1717030858387858
1105	25	0.421191660147187
1106	1	-0.8778348699627507
1106	2	-1.0156001391269776
1106	3	-0.9605960244545813
1106	4	-1.0806009143012716
1106	5	-0.8077422913471821
1106	6	-1.0224493750539096
1106	7	-0.7210805586345259
1106	8	-0.9296444957847783
1106	9	-1.0131671340730577
1106	10	-0.893653435184838
1106	11	-0.9668313231813457
1106	12	-0.884439773163848
1106	13	-0.9389306120327134
1106	14	-1.2922463543053837
1106	15	-0.8313567284245877
1106	16	-0.9460543204597656
1106	17	-0.7999719211000027
1106	18	-0.9252880865923316
1106	19	-0.7941973822386501
1106	20	-0.8640284327299952
1106	21	-0.6819711489573805
1106	22	-1.108072538007768
1106	23	-0.6607889627221581
1106	24	-0.7389398396952558
1106	25	-0.5339055900204883
1107	1	-0.01577137789925942
1107	2	-0.06861601214285074
1107	3	0.01654683268827739
1107	4	0.16749432379396012
1107	5	-0.12663118023606756
1107	6	-0.12213191473644969
1107	7	0.019236901682937457
1107	8	-0.05218417832445965
1107	9	0.19222969132376733
1107	10	-0.06714549867690067
1107	11	-0.11270433905436761
1107	12	-0.16650326522734105
1107	13	-0.047184580286682376
1107	14	0.14219809013906115
1107	15	-0.08484879191665584
1107	16	-0.14716543157087614
1107	17	-0.3087020798301623
1107	18	-0.030049991354232475
1107	19	-0.1891180171592879
1107	20	-0.23656811526967658
1107	21	-0.2876854346716655
1107	22	-0.17600904594426936
1107	23	0.16921103727784015
1107	24	-0.21401920477462255
1107	25	-0.38866749478238916
1108	1	0.04010232660785107
1108	2	-0.11358588440122475
1108	3	0.21180352104691735
1108	4	0.07582404179829894
1108	5	0.10075373179092395
1108	6	0.20118454706157252
1108	7	-0.10156811157327184
1108	8	0.17318579034268566
1108	9	0.44450252815191504
1108	10	0.3579786276185928
1108	11	0.43654126229535706
1108	12	0.14888839541377763
1108	13	0.2442012958280415
1108	14	0.3652012234241866
1108	15	0.36662385209395154
1108	16	0.23670056023437525
1108	17	0.11506268276323084
1108	18	0.37291457842161435
1108	19	0.19862360732854256
1108	20	0.141224123824081
1108	21	0.14265440625385395
1108	22	0.09681332089110839
1108	23	0.05674538290329423
1108	24	-0.011406527020945134
1108	25	0.11747856450900684
1109	1	0.24320015561772834
1109	2	0.6727705810619256
1109	3	0.4619738299334557
1109	4	0.5818744890832193
1109	5	0.5805497196720992
1109	6	0.4918166619394029
1109	7	0.15682184199514637
1109	8	0.2317093019326535
1109	9	0.349465687650671
1109	10	0.2866102405333848
1109	11	0.23832412742864761
1109	12	0.42730757040167333
1109	13	0.5488301120916432
1109	14	0.47565998178276914
1109	15	0.5570336690567146
1109	16	0.5250200597055255
1109	17	0.4178360285261249
1109	18	0.43014284611133036
1109	19	0.5545826256322641
1109	20	0.42693969005631516
1109	21	0.47267269205009654
1109	22	0.7432655178757486
1109	23	0.6025719188205443
1109	24	0.4053931000922262
1109	25	0.3503132214710547
1110	1	-0.07658960198108034
1110	2	-0.10274897355614598
1110	3	-0.17946432491283915
1110	4	-0.20798684775415666
1110	5	0.03542367796941903
1110	6	-0.3373527422585312
1110	7	-0.2556491978056741
1110	8	-0.20260723736677022
1110	9	-0.47963455786265996
1110	10	-0.22281263024361664
1110	11	-0.5036015961440938
1110	12	-0.5135790439349179
1110	13	-0.46859787642801365
1110	14	-0.170558767136626
1110	15	-0.46776836420860946
1110	16	-0.5935438602087189
1110	17	-0.3564473146046865
1110	18	-0.22223967057320237
1110	19	-0.15412963966044657
1110	20	-0.4641180920246697
1110	21	-0.31928933983206065
1110	22	-0.07928194227156382
1110	23	0.1462542729821692
1110	24	0.10460469108776493
1110	25	0.05205774929013529
1111	1	0.5348988875078469
1111	2	0.9031968533542241
1111	3	0.636087993282068
1111	4	0.5965631497048918
1111	5	0.30919428085255163
1111	6	0.5503287240399786
1111	7	0.568296730742766
1111	8	0.47328590714562324
1111	9	0.6541550174596154
1111	10	0.614896786523282
1111	11	0.5367063671984482
1111	12	0.6201004234816097
1111	13	0.8805797022144404
1111	14	0.38150533260419905
1111	15	0.3352231828921646
1111	16	0.4739861653701922
1111	17	0.7710999894455881
1111	18	0.8142145160681693
1111	19	0.47888918032159467
1111	20	0.550170526205811
1111	21	0.6041094371232063
1111	22	0.41525501163557266
1111	23	0.5186307403817594
1111	24	0.29735281501844923
1111	25	0.6403473500219263
1112	1	0.16133394385536093
1112	2	0.29450578701101193
1112	3	-0.0620370016470595
1112	4	-0.03759585656676023
1112	5	0.04032050090398798
1112	6	-0.09764739013168366
1112	7	-0.09186975607703118
1112	8	-0.25825409593746684
1112	9	-0.532749155258287
1112	10	-0.34312857594254353
1112	11	-0.4568637690254178
1112	12	-0.39561927502471406
1112	13	-0.3202551659023598
1112	14	-0.4767820924483388
1112	15	-0.32946246370467236
1112	16	-0.6960543204597656
1112	17	-0.1377379293068941
1112	18	-0.1277787822694023
1112	19	0.1405769282871674
1112	20	0.18207806598172027
1112	21	0.09069799886206908
1112	22	-0.09752763868530678
1112	23	-0.207448949362103
1112	24	0.18984630402074743
1112	25	0.09763845044138719
1113	1	0.14243789933914286
1113	2	0.02378959735013808
1113	3	0.0020484508112552646
1113	4	-0.12939888008953204
1113	5	0.17073667305411533
1113	6	0.04153583067886757
1113	7	0.34970076360311
1113	8	-0.05091125491561854
1113	9	-0.12509501202035977
1113	10	-0.07564603805122516
1113	11	-0.0351854609529596
1113	12	-0.1545507301680118
1113	13	-0.2947789707936934
1113	14	-0.03674473618240626
1113	15	0.0675568175763317
1113	16	-0.11158830104228912
1113	17	0.14674560086023547
1113	18	-0.03749335705003176
1113	19	0.24154001951816895
1113	20	0.237843966715225
1113	21	0.0569100345839928
1113	22	-0.0827835874760936
1113	23	0.16655731559499287
1113	24	-0.022088244688326597
1113	25	0.2352807252823368
1114	1	-0.18626472839261154
1114	2	0.040770517243679194
1114	3	-0.14528070913926427
1114	4	-0.3708840545844154
1114	5	-0.21437034797524035
1114	6	-0.10417395677848873
1114	7	-0.20434954190351107
1114	8	-0.3286148947551766
1114	9	-0.5293833502892795
1114	10	-0.471710063241467
1114	11	-0.4316318379818611
1114	12	-0.39660193532601085
1114	13	-0.2982613713634734
1114	14	-0.38330169536072844
1114	15	-0.4451919922598577
1114	16	-0.184471308876752
1114	17	-0.4394185105465951
1114	18	-0.5967166580209025
1114	19	-0.44413303217429956
1114	20	-0.4271558458574063
1114	21	-0.1582903252765604
1114	22	-0.2303376602728875
1114	23	-0.756477508410704
1114	24	-0.4561213068767245
1114	25	-0.35520546132035946
1115	1	0.1951408572931328
1115	2	0.06283232335217903
1115	3	-0.10021062515867385
1115	4	0.07169665842431527
1115	5	0.054968473892763825
1115	6	0.22472498106986194
1115	7	0.1720081943764029
1115	8	-0.1340172954883201
1115	9	0.15014581756673806
1115	10	-0.002260068499644774
1115	11	0.12782311446919437
1115	12	0.05322003784226581
1115	13	-0.37013313195119224
1115	14	-0.18102715845584072
1115	15	-0.23425648755002726
1115	16	-0.19799984575159613
1115	17	-0.47216943823915614
1115	18	-0.40410965412985433
1115	19	-0.47300042096386363
1115	20	-0.06600175124027174
1115	21	-0.2047801169025334
1115	22	0.1963558481326828
1115	23	0.11930182845812709
1115	24	0.1289034065526593
1115	25	0.02313535865470584
1116	1	-0.12436340504090637
1116	2	-0.14722500281290962
1116	3	0.2349692245457824
1116	4	-0.19670414948848247
1116	5	-0.009523461830639235
1116	6	-0.33142792830727075
1116	7	-0.4041049134146064
1116	8	-0.29433006466882006
1116	9	-0.49010279383314526
1116	10	-0.5002328608482358
1116	11	-0.3788451363402032
1116	12	-0.49643541111368705
1116	13	-0.42724395263613246
1116	14	-0.3927552601578057
1116	15	-0.40918297342641097
1116	16	0.006235755876112137
1116	17	-0.36069529441879666
1116	18	-0.49557161985296005
1116	19	-0.2652079238598759
1116	20	-0.27247990492192287
1116	21	-0.602291032636046
1116	22	-0.35434662015970986
1116	23	-0.2464123723913687
1116	24	-0.2195832420943873
1116	25	-0.47390922506229316
1201	1	-0.13109301532616158
1201	2	-0.29297539544960216
1201	3	-0.23343721834840636
1201	4	-0.39681189630172753
1201	5	-0.47034197236791186
1201	6	-0.4382616329714324
1201	7	-0.3641655438247753
1201	8	-0.5736408503074486
1201	9	-0.5940967307921312
1201	10	-0.6859865406758345
1201	11	-0.3694401178954081
1201	12	-0.1975293152007609
1201	13	-0.40963008799535316
1201	14	-0.2009385393133627
1201	15	-0.305189036467425
1201	16	-0.042944272612878365
1201	17	-0.4366659288466437
1201	18	-0.3646592417529604
1201	19	-0.42964964926986227
1201	20	-0.5017249467422928
1201	21	-0.5683233932043592
1201	22	-0.7016018157475656
1201	23	-0.5632610520363528
1201	24	-0.4906486571935531
1201	25	-0.5186515458190755
1202	1	-0.12807514956082855
1202	2	-0.048758723661709524
1202	3	-0.33816264743404645
1202	4	-0.21727631840786898
1202	5	-0.13174840755512918
1202	6	-0.4433755436864999
1202	7	-0.5410892960744533
1202	8	-0.5522963088035624
1202	9	-0.3328263739157862
1202	10	-0.222639892152948
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2209	25	0.47969768568469817
2210	1	-0.06218647223888141
2210	2	0.31222256228063117
2210	3	-0.0028242364941135634
2210	4	0.2014146592237296
2210	5	0.3087147317136143
2210	6	0.15484921733902013
2210	7	0.11764361027832138
2210	8	0.22534202712627405
2210	9	-0.173304899871205
2210	10	-0.08324612760771544
2210	11	0.10712794606094178
2210	12	-0.003232824975768267
2210	13	0.08086573417872245
2210	14	-0.024426647806436108
2210	15	-0.13164124474684513
2210	16	0.33539222042073646
2210	17	0.24182202918451168
2210	18	0.042696339882660084
2210	19	0.09394277948641871
2210	20	0.13990987215171202
2210	21	-0.20374413488131182
2210	22	-0.313074035462094
2210	23	-0.04726695014354476
2210	24	0.08099127740567269
2210	25	-0.14548091197316637
2211	1	0.1658834684364372
2211	2	0.24731779400372034
2211	3	0.13768158446132617
2211	4	0.14366453655991762
2211	5	-0.06057917077980335
2211	6	0.05039763609097747
2211	7	0.2836813461273806
2211	8	0.25708296115544726
2211	9	0.394735601489252
2211	10	0.15715710179591014
2211	11	0.3124493252482381
2211	12	-0.01681059383254535
2211	13	0.21811092798754572
2211	14	0.27122882299957496
2211	15	0.12299990784693904
2211	16	0.15288184975300112
2211	17	0.21801186816038154
2211	18	0.37775142708547804
2211	19	0.01745408685962957
2211	20	0.1039046979690994
2211	21	0.2784138561084717
2211	22	0.2714614032283009
2211	23	0.11119685288068126
2211	24	0.08735195361781223
2211	25	0.22693534209704325
2212	1	0.1297304142154374
2212	2	0.02840907746288579
2212	3	0.15393930580655635
2212	4	0.1486233560520276
2212	5	0.14938136456679985
2212	6	0.1605383361596111
2212	7	0.18166521709512296
2212	8	-0.12876123925636307
2212	9	0.13902180601910175
2212	10	0.1616269027567938
2212	11	-0.03530290536260594
2212	12	-0.01929000357859678
2212	13	-0.1151594906809521
2212	14	-0.06509581667097564
2212	15	0.07862791059231
2212	16	-0.06500593336298977
2212	17	-0.15431139882657874
2212	18	-0.10276504511767826
2212	19	0.19476191115612806
2212	20	0.22905912487369307
2212	21	0.26726080188747403
2212	22	0.4052723160628986
2212	23	0.12321641362192537
2212	24	0.047638501318566284
2212	25	-0.05466211843830848
2213	1	0.5767395582337258
2213	2	0.7609465425378161
2213	3	0.7049457828195109
2213	4	0.39922660725853376
2213	5	0.2582682073404854
2213	6	0.5791629234087807
2213	7	0.39431126738722355
2213	8	0.435278638823398
2213	9	0.7200162180079275
2213	10	0.4086900218830323
2213	11	0.5150209452850945
2213	12	0.04340049680240554
2213	13	0.1557638011656337
2213	14	0.40197936747939167
2213	15	0.6543200619766125
2213	16	0.5362291441071605
2213	17	0.3930914459791097
2213	18	0.5450156254436678
2213	19	0.6452476871276787
2213	20	0.7393461454477332
2213	21	0.6128919931498586
2213	22	0.8214362733908089
2213	23	0.8948540818972646
2213	24	1.153636088313739
2213	25	0.9020149199157714
2214	1	0.5987524316245505
2214	2	0.6086062100793725
2214	3	0.5890071501485963
2214	4	0.5392403555399952
2214	5	0.5009878673829746
2214	6	0.73350300589847
2214	7	0.3047130921591261
2214	8	0.6536888375485574
2214	9	0.5459598500539258
2214	10	0.7059497394183367
2214	11	0.7855496291996005
2214	12	0.5671475284234546
2214	13	0.7507519276498229
2214	14	0.5609282488692173
2214	15	0.9337226366547711
2214	16	0.6710091716037283
2214	17	0.572647126519044
2214	18	0.8437595324552873
2214	19	0.8188978558565907
2214	20	0.8808128371112787
2214	21	0.8414415494553182
2214	22	1.1220861921509666
2214	23	1.0411951642619712
2214	24	1.028917303161883
2214	25	0.8752213941064966
2215	1	0.18645084432296244
2215	2	0.2148760513492114
2215	3	0.007023023164467901
2215	4	0.2560657523653944
2215	5	0.26416247055757935
2215	6	0.175645863041332
2215	7	0.19606229850833046
2215	8	0.34940312326283873
2215	9	0.12683286592693577
2215	10	0.2344418029104034
2215	11	-0.04635513270515901
2215	12	0.28746498874091486
2215	13	0.002974149872045473
2215	14	0.08442031236128145
2215	15	-0.04421387128173393
2215	16	0.022040917635472113
2215	17	0.293837602709516
2215	18	0.11756905626481284
2215	19	0.09056452252325187
2215	20	-0.3130760517776139
2215	21	-0.06673305371928961
2215	22	-0.08426301419824256
2215	23	-0.10221753415073209
2215	24	-0.0070350777904977235
2215	25	-0.07438178049667954
2216	1	0.3366713862259232
2216	2	0.2866412867439365
2216	3	0.3306993216611822
2216	4	0.1483260818279888
2216	5	0.4014889625910669
2216	6	0.2641650816928731
2216	7	0.2488987374317304
2216	8	-0.1491512020589738
2216	9	-0.22080958613895518
2216	10	-0.27240489796276535
2216	11	-0.2261300899470342
2216	12	-0.22849864434937217
2216	13	-0.2999802114566066
2216	14	-0.023027702761595492
2216	15	-0.16713402611114958
2216	16	-0.29718853596071426
2216	17	-0.25455379165541103
2216	18	-0.06552573174491272
2216	19	-0.1425185632630459
2216	20	0.023238648015350805
2216	21	0.21404109336866384
2216	22	0.055803598458162185
2216	23	0.19122742039063212
2216	24	-0.12329036629531487
2216	25	-0.3666133044862363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94.0
3	49.0
4	7.0
5	22.0
6	18.0
7	32.0
8	44.0
9	76.0
10	159.0
11	270.0
12	441.0
13	791.0
14	1417.0
15	2310.0
16	3090.0
17	3391.0
18	3272.0
19	3024.0
20	2915.0
21	2662.0
22	2428.0
23	2419.0
24	2328.0
25	2172.0
26	2142.0
27	2141.0
28	2271.0
29	2414.0
30	2912.0
31	3612.0
32	4705.0
33	6424.0
34	9155.0
35	13859.0
36	24409.0
37	52388.0
38	138512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65556681724515	23.682550123841295	10.485565273424127	22.176317785489424
2	11.41209736659388	29.97608433876383	30.938748402547873	27.673069892094414
3	21.47599694177297	36.568280283824926	24.110699770632973	17.845023003769132
4	13.306697797368649	29.034746962408338	24.65833383652356	33.000221403699456
5	9.884253281055573	45.663438995676685	31.204322641882804	13.247985081384936
6	29.557269830622168	34.69059198390072	16.969981552909612	18.7821566325675
7	19.04024040944734	40.923268870867126	20.8161444603197	19.22034625936584
8	26.01119619503785	38.83516638323992	19.675182885720325	15.478454536001903
9	25.51030675422768	24.598361920349888	17.518463612763874	32.37286771265856
10	17.624607170047994	38.43042423957848	26.446469477490048	17.49849911288348
11	22.931850425788102	34.620815487350455	15.319315921691212	27.128018165170232
12	23.25001844733788	39.789901591837555	23.687388058199673	13.272691902624889
13	28.443493368931584	27.737453964889212	18.99900047628311	24.82005218989609
14	19.99134704390872	36.80591889027515	21.497565098402223	21.705168967413908
15	34.52561819383098	26.581077047426444	18.86291910238222	20.030385656360362
16	14.840776080358193	34.07609880435329	32.05205171633156	19.031073398956956
17	25.245193404263883	36.33021614586827	23.05824265761475	15.366347792253096
18	24.965201262481425	28.53328771763885	27.157437873771983	19.34407314610774
19	21.365973417046494	38.27194032754116	27.210802217593848	13.151284037818495
20	23.059712256761696	30.923844314068827	24.820453988923664	21.195989440245814
21	17.675964686309236	35.28048355068963	28.45589813771266	18.587653625288468
22	21.624676731728425	33.57327581869472	17.557953489620196	27.24409395995666
23	15.761472998493858	34.084390818246774	26.023018117291098	24.131118065968273
24	20.615862536315024	41.519896942493105	20.123387210402367	17.740853310789504
25	20.943767758123872	29.323086315803597	18.63751790488522	31.09562802118731
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14810.0
1	14810.0
2	9851.5
3	4893.0
4	4893.0
5	4893.0
6	4696.0
7	4499.0
8	4499.0
9	4499.0
10	4081.0
11	3663.0
12	3663.0
13	3663.0
14	3508.0
15	3353.0
16	3353.0
17	3353.0
18	3744.0
19	4135.0
20	4135.0
21	4135.0
22	4858.5
23	5582.0
24	5582.0
25	5582.0
26	6411.5
27	7241.0
28	7241.0
29	7241.0
30	7907.0
31	8573.0
32	8573.0
33	8573.0
34	8961.0
35	9349.0
36	9349.0
37	9349.0
38	9351.5
39	9354.0
40	9354.0
41	9354.0
42	13328.5
43	17303.0
44	17303.0
45	17303.0
46	58403.0
47	99503.0
48	99503.0
49	99503.0
50	82561.5
51	65620.0
52	65620.0
53	65620.0
54	44098.5
55	22577.0
56	22577.0
57	22577.0
58	15063.5
59	7550.0
60	7550.0
61	7550.0
62	6381.5
63	5213.0
64	5213.0
65	5213.0
66	3995.0
67	2777.0
68	2777.0
69	2777.0
70	2049.5
71	1322.0
72	1322.0
73	1322.0
74	978.5
75	635.0
76	635.0
77	635.0
78	462.5
79	290.0
80	290.0
81	290.0
82	187.5
83	85.0
84	85.0
85	85.0
86	60.5
87	36.0
88	36.0
89	36.0
90	22.0
91	8.0
92	8.0
93	8.0
94	4.5
95	1.0
96	1.0
97	1.0
98	2.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13841642228739004
2	0.08177628822790113
3	0.05462924172601592
4	0.09283619606200251
5	0.0750733137829912
6	0.0754084625052367
7	0.07272727272727272
8	0.07976539589442816
9	0.07406786761625471
10	0.07306242144951823
11	0.0744030163385002
12	0.07674905739421868
13	0.07808965228320068
14	0.07071638039379975
15	0.06971093422706326
16	0.07038123167155425
17	0.08144113950565564
18	0.07775450356095517
19	0.07172182656053624
20	0.08780896522832006
21	0.08278173439463761
22	0.08311688311688312
23	0.08780896522832006
24	0.09685798072894847
25	0.09116045245077503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	298375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.57980728948471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21379874556858	19.224801005446164
2	12.23343332424325	6.013908671973188
3	4.139623670575402	3.0525345622119815
4	1.8898281974365965	1.8580645161290321
5	0.9599127352058904	1.1797235023041475
6	0.5890373602399782	0.8687054880603267
7	0.35178620125443144	0.6052785923753665
8	0.21543496045814017	0.42362798491830755
9	0.1649850013635124	0.3649769585253456
>10	0.9544586855740387	4.698449937159615
>50	0.1104445050449959	1.9562630917469626
>100	0.109080992637033	6.21767909509845
>500	0.017725661303517864	2.8464180980310014
>1k	0.03681483501499864	19.374947633012148
>5k	0.009544586855740387	14.404356933389192
>10k+	0.004090537223888737	16.91026392961877
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	18364	6.154671135316296	No Hit
CCCATGTACTCTGCGTTGATACCAC	17955	6.0175953079178885	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	14137	4.737997486384583	No Hit
GAGTACATGGGAAGCAGTGGTATCA	8744	2.9305404273146207	No Hit
CATGTACTCTGCGTTGATACCACTG	7149	2.395978215333054	No Hit
GCGTTGATACCACTGCTTCCCATGT	5900	1.977377461248429	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5637	1.8892333472978633	No Hit
GGTATCAACGCAGAGTACATGGGAA	5334	1.787683284457478	No Hit
TATCAACGCAGAGTACATGGGAAGC	5204	1.7441139505655634	No Hit
GTATCAACGCAGAGTACATGGGAAG	5011	1.6794302471721825	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4662	1.5624633431085044	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3478	1.1656472559698368	No Hit
GTGGTATCAACGCAGAGTACATGGG	3392	1.136824465856724	No Hit
GTACTCTGCGTTGATACCACTGCTT	3160	1.0590699622957687	No Hit
ATACCACTGCTTCCCATGTACTCTG	2730	0.9149560117302052	No Hit
GATACCACTGCTTCCCATGTACTCT	2725	0.9132802681189779	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2543	0.8522832006702974	No Hit
CAGTGGTATCAACGCAGAGTACATG	2460	0.8244658567239213	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2354	0.7889400921658987	No Hit
CATGGGAAGCAGTGGTATCAACGCA	2248	0.753414327607876	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	2162	0.7245915374947633	No Hit
TACCACTGCTTCCCATGTACTCTGC	2152	0.7212400502723083	No Hit
GTTGATACCACTGCTTCCCATGTAC	2098	0.7031420192710515	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2095	0.702136573104315	No Hit
ACATGGGAAGCAGTGGTATCAACGC	2033	0.6813573523250942	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1877	0.6290741516547969	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1868	0.6260578131545873	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1638	0.5489736070381231	No Hit
CCATGTACTCTGCGTTGATACCACT	1615	0.5412651864264767	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	1609	0.5392542940930038	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1473	0.49367406786761625	No Hit
TTCCCATGTACTCTGCGTTGATACC	1410	0.47255969836614997	No Hit
TGATACCACTGCTTCCCATGTACTC	1331	0.44608294930875575	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1226	0.4108923334729786	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1201	0.40251361541684116	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1149	0.3850858818600754	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1121	0.3757017176372015	No Hit
TATCAACGCAGAGTACTTTTTTTTT	908	0.3043150397989107	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	853	0.28588186007540844	No Hit
ACCATGTACTCTGCGTTGATACCAC	839	0.2811897779639715	No Hit
CTCTGCGTTGATACCACTGCTTCCC	663	0.22220360284876411	No Hit
AAAAAGTACTCTGCGTTGATACCAC	653	0.21885211562630916	No Hit
TCAACGCAGAGTACATGGGAAGCAG	647	0.21684122329283623	No Hit
CTGCGTTGATACCACTGCTTCCCAT	633	0.21214914118139921	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	615	0.2061164641809803	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	570	0.19103477167993296	No Hit
TCCATGTACTCTGCGTTGATACCAC	569	0.19069962295768747	No Hit
TCCCATGTACTCTGCGTTGATACCA	535	0.1793045664013406	No Hit
CCCCATGTACTCTGCGTTGATACCA	507	0.16992040217846668	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	501	0.16790950984499373	No Hit
GTACATGGAAAGCAGTGGTATCAAC	482	0.16154168412232928	No Hit
AAAGTACTCTGCGTTGATACCACTG	477	0.15986594051110178	No Hit
ATGTACTCTGCGTTGATACCACTGC	476	0.15953079178885632	No Hit
AGTGGTATCAACGCAGAGTACATGG	472	0.15819019689987432	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	468	0.15684960201089235	No Hit
AACGCAGAGTACATGGGAAGCAGTG	464	0.15550900712191035	No Hit
GAGTACATGGTAAGCAGTGGTATCA	429	0.14377880184331795	No Hit
TTGATACCACTGCTTCCCATGTACT	418	0.1400921658986175	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	415	0.13908671973188103	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	412	0.13808127356514452	No Hit
GCTTACCATGTACTCTGCGTTGATA	407	0.13640552995391705	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	373	0.12501047339757018	No Hit
GTACATGGGTAAGCAGTGGTATCAA	370	0.12400502723083368	No Hit
CAACGCAGAGTACATGGGAAGCAGT	353	0.11830749895266025	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	348	0.11663175534143276	No Hit
GAGTACATGGAAAGCAGTGGTATCA	345	0.11562630917469627	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	338	0.1132802681189778	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	319	0.10691244239631335	No Hit
GCGTTGATACCACTGCTTACCATGT	304	0.1018852115626309	No Hit
CGTTGATACCACTGCTTCCCATGTA	302	0.10121491411813992	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	301	0.10087976539589444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	3.3514872224549644E-4	0.0	0.0	0.0	0.0
10	3.3514872224549644E-4	0.0	0.0	0.0	0.0
11	3.3514872224549644E-4	0.0	0.0	0.0	0.0
12	3.3514872224549644E-4	0.0	0.0	0.0	0.0
13	3.3514872224549644E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGA	45	3.5124867E-5	16.895006	2
GTACAAA	55	1.1112645E-5	15.561535	1
GTAAAAA	65	3.3142987E-6	14.630503	1
ACATGAA	50	0.0014905167	13.304818	3
GAACAAA	255	0.0	12.6797695	1
ACGCAAA	60	0.005811867	11.094798	1
TACAAAA	125	2.201923E-7	10.643854	2
GAGAACA	90	9.3564086E-5	10.566475	1
GAAAACA	155	3.5361154E-9	10.430134	1
GGAAAAA	130	4.2777036E-5	8.778301	1
CAGAAAA	130	4.3116048E-5	8.772408	4
TGGTAAG	335	0.0	8.222718	6
CATGGTA	350	0.0	8.145807	4
ATGGTAA	350	0.0	7.8703165	5
AAAGTAC	195	1.3150966E-6	7.802935	1
AAGTACT	195	1.3285025E-6	7.7976956	2
AGTACTC	195	1.3285025E-6	7.7976956	3
GAAAAAA	760	0.0	7.6328874	1
AAAAGTA	140	9.14764E-4	7.4669895	2
CCATGTA	5170	0.0	7.4630504	2
>>END_MODULE