FastQCFastQC Report
Wed 25 May 2016
SRR1294903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298375
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC191806.428152492668622No Hit
GTACATGGGAAGCAGTGGTATCAAC184946.198240469208211No Hit
GAGTACATGGGAAGCAGTGGTATCA90193.0227063259321323No Hit
CATGTACTCTGCGTTGATACCACTG74732.504566401340595No Hit
ACGCAGAGTACATGGGAAGCAGTGG61512.0614997905320487No Hit
GCGTTGATACCACTGCTTCCCATGT60552.029325513196481No Hit
GTATCAACGCAGAGTACTTTTTTTT58371.9562630917469626No Hit
TATCAACGCAGAGTACATGGGAAGC54381.8225387515710096No Hit
GGTATCAACGCAGAGTACATGGGAA53031.7772936740678678No Hit
GTATCAACGCAGAGTACATGGGAAG51971.7417679095098448No Hit
ACTCTGCGTTGATACCACTGCTTCC50831.7035609551738586No Hit
GGTATCAACGCAGAGTACTTTTTTT48241.6167574361122747No Hit
TATCAACGCAGAGTACTTTTTTTTT40071.3429409300377042No Hit
GCTTCCCATGTACTCTGCGTTGATA35931.2041893590280688No Hit
GTGGTATCAACGCAGAGTACATGGG34341.1509007121910348No Hit
GTACTCTGCGTTGATACCACTGCTT30271.0144951822371178No Hit
GATACCACTGCTTCCCATGTACTCT28360.9504817762882278No Hit
ATACCACTGCTTCCCATGTACTCTG27580.9243401759530793No Hit
CATGGGAAGCAGTGGTATCAACGCA25840.8660242982823628No Hit
GGGAAGCAGTGGTATCAACGCAGAG25720.8620025136154168No Hit
CAGTGGTATCAACGCAGAGTACATG25340.8492668621700881No Hit
CTGCTTCCCATGTACTCTGCGTTGA24520.8217846669459572No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24500.8211143695014663No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23670.7932970255550901No Hit
GCAGAGTACATGGGAAGCAGTGGTA22750.7624633431085044No Hit
ACATGGGAAGCAGTGGTATCAACGC22460.752744030163385No Hit
GCAGTGGTATCAACGCAGAGTACAT22150.7423544197737746No Hit
TACCACTGCTTCCCATGTACTCTGC21390.7168831168831169No Hit
CTTCCCATGTACTCTGCGTTGATAC20110.6739840804356934No Hit
GTTGATACCACTGCTTCCCATGTAC20060.6723083368244658No Hit
CCACTGCTTCCCATGTACTCTGCGT18870.6324256388772518No Hit
CCATGTACTCTGCGTTGATACCACT17940.6012568077084206No Hit
ATGGGAAGCAGTGGTATCAACGCAG17510.5868454126518642No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17490.5861751152073733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16430.5506493506493506No Hit
AAGCAGTGGTATCAACGCAGAGTAC15930.5338919145370759No Hit
TTCCCATGTACTCTGCGTTGATACC14260.47792207792207786No Hit
TGATACCACTGCTTCCCATGTACTC14090.47222454964390453No Hit
ATCAACGCAGAGTACATGGGAAGCA12660.42429828236279843No Hit
GTACATGGTAAGCAGTGGTATCAAC11540.3867616254713029No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10360.3472140762463343No Hit
GGAAGCAGTGGTATCAACGCAGAGT9230.3093422706325932No Hit
ACCATGTACTCTGCGTTGATACCAC9200.30833682446585675No Hit
GTGGTATCAACGCAGAGTACTTTTT8980.3009635525764558No Hit
TCAACGCAGAGTACATGGGAAGCAG7200.24130708001675744No Hit
TGGGAAGCAGTGGTATCAACGCAGA6840.22924172601591955No Hit
ATCAACGCAGAGTACTTTTTTTTTT6780.22723083368244656No Hit
CTGCGTTGATACCACTGCTTCCCAT6690.2242144951822371No Hit
CTCTGCGTTGATACCACTGCTTCCC6580.22052785923753665No Hit
AAAAAGTACTCTGCGTTGATACCAC6220.2084625052366988No Hit
CCCCATGTACTCTGCGTTGATACCA5990.20075408462505237No Hit
TCCATGTACTCTGCGTTGATACCAC5590.1873481357352325No Hit
GGTAAGCAGTGGTATCAACGCAGAG5300.1776288227901131No Hit
AACGCAGAGTACATGGGAAGCAGTG5260.17628822790113113No Hit
TCCCATGTACTCTGCGTTGATACCA5060.16958525345622122No Hit
AGTGGTATCAACGCAGAGTACATGG5040.1689149560117302No Hit
AAAGTACTCTGCGTTGATACCACTG4810.16120653540008378No Hit
GAGTACATGGTAAGCAGTGGTATCA4610.15450356095517387No Hit
GTACATGGAAAGCAGTGGTATCAAC4380.14679514034352745No Hit
ATGTACTCTGCGTTGATACCACTGC4340.14545454545454545No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4340.14545454545454545No Hit
ACTGCTTCCCATGTACTCTGCGTTG4080.13674067867616255No Hit
GCTTACCATGTACTCTGCGTTGATA4050.13573523250942607No Hit
TTGATACCACTGCTTCCCATGTACT4020.13472978634268956No Hit
CAACGCAGAGTACATGGGAAGCAGT3740.12534562211981565No Hit
GTACATGGGTAAGCAGTGGTATCAA3680.12333472978634269No Hit
AACGCAGAGTACTTTTTTTTTTTTT3550.11897779639715124No Hit
CAACGCAGAGTACTTTTTTTTTTTT3440.11529116045245076No Hit
GAGTACATGGAAAGCAGTGGTATCA3420.11462086300795978No Hit
CGTTGATACCACTGCTTCCCATGTA3280.10992878089652283No Hit
CGCAGAGTACATGGGAAGCAGTGGT3250.10892333472978634No Hit
ACTCTGCGTTGATACCACTGCTTAC3240.10858818600754083No Hit
GCGTTGATACCACTGCTTACCATGT3200.10724759111855886No Hit
ACGCAGAGTACATGGTAAGCAGTGG3130.1049015500628404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATAA250.006012322419.0046942
GTACAAA400.00524556714.2583021
GAACAAA1152.5465852E-1114.0516591
ATGGCAA551.9504948E-413.8192795
AAAAAGT1400.013.5793341
AAAAGTA1400.013.5747812
CATGGCA701.08440916E-412.2152554
AGTACTC1650.012.0918695
TGGCAAG657.9848064E-411.6932356
AAGTACT1751.8189894E-1211.4009064
ACATGGC855.2850028E-511.1773583
AAAGTAC1701.0913936E-1111.1773583
GGCAAGC700.001486386310.8561857
CAAGCAG700.001486386310.8561859
GCAAGCA800.00450069559.4991628
ACACAAA950.00180488939.0052431
CACAAAA950.00181040929.0022242
ACAAAAA4550.08.1435033
CCCCATG2701.2732926E-108.0973071
AATCAAC1605.738122E-57.71806919
Produced by FastQC (version 0.11.5)