##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294903_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 298375 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.503654796816086 33.0 31.0 34.0 28.0 34.0 2 31.67440636782572 33.0 31.0 34.0 28.0 34.0 3 31.826721407624632 33.0 31.0 34.0 29.0 34.0 4 35.21011143695014 37.0 35.0 37.0 33.0 37.0 5 35.497498114788435 37.0 35.0 37.0 33.0 37.0 6 35.19180226225387 37.0 35.0 37.0 33.0 37.0 7 35.20522832006703 37.0 35.0 37.0 32.0 37.0 8 35.21888898198576 37.0 35.0 37.0 33.0 37.0 9 36.79631001256808 39.0 37.0 39.0 33.0 39.0 10 36.89586929199832 39.0 37.0 39.0 33.0 39.0 11 36.89221617092585 39.0 37.0 39.0 33.0 39.0 12 36.92392459153749 39.0 37.0 39.0 33.0 39.0 13 36.76124340175953 39.0 37.0 39.0 32.0 39.0 14 38.04127021365731 40.0 38.0 41.0 33.0 41.0 15 37.94928864683703 40.0 38.0 41.0 32.0 41.0 16 37.842795140343526 40.0 38.0 41.0 32.0 41.0 17 38.009930456640134 40.0 38.0 41.0 33.0 41.0 18 38.01277251780478 40.0 38.0 41.0 33.0 41.0 19 37.954888981985754 40.0 38.0 41.0 32.0 41.0 20 38.04348554671135 40.0 38.0 41.0 32.0 41.0 21 37.95794553833264 40.0 38.0 41.0 32.0 41.0 22 38.0722982823628 40.0 38.0 41.0 32.0 41.0 23 38.00844239631336 40.0 38.0 41.0 32.0 41.0 24 38.01638877251781 40.0 38.0 41.0 32.0 41.0 25 36.97621114369502 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23672799920744225 1101 2 -0.4883059199007711 1101 3 -0.4759026269543014 1101 4 -0.3540302519189211 1101 5 -0.31134018241895944 1101 6 -0.5579935039739041 1101 7 -0.04213554253599483 1101 8 -0.36018642905674625 1101 9 -0.385756798771304 1101 10 -0.2927855228101066 1101 11 -0.22791415979077811 1101 12 -0.2689786547104518 1101 13 -0.09451005982399607 1101 14 -0.18740509669483885 1101 15 -0.3712726101999735 1101 16 -0.3038105608351884 1101 17 -0.31416597600402696 1101 18 -0.30783202062636406 1101 19 -0.3761860257546559 1101 20 -0.5168970049151369 1101 21 -0.21813124075577406 1101 22 -0.2118701634988085 1101 23 -0.3065310118339539 1101 24 -0.3738230122299342 1101 25 -0.22723159819877026 1102 1 -0.32865070825946674 1102 2 -0.21827658115105564 1102 3 -0.15118284962788664 1102 4 -0.3269859428840931 1102 5 -0.2763217516659324 1102 6 -0.552179488004171 1102 7 -0.43260421025584606 1102 8 -0.3223147242926885 1102 9 -0.6749154322088842 1102 10 -0.2302821644367441 1102 11 -0.4700609395137221 1102 12 -0.6401729460130667 1102 13 -0.6909088088970847 1102 14 -0.338558765113234 1102 15 -0.41638765463797256 1102 16 0.04695944700997501 1102 17 -0.35562173682336606 1102 18 -0.12963941657052658 1102 19 -0.16195941884016918 1102 20 -0.5007875595572173 1102 21 -0.6989428386966878 1102 22 -0.49123309350493116 1102 23 -0.3700284487233958 1102 24 -0.5965825741905775 1102 25 -0.351727842194002 1103 1 -0.1064284860372453 1103 2 -0.07088429090162052 1103 3 -0.11036652309727657 1103 4 -0.14331247349633713 1103 5 -0.18335123012398213 1103 6 -0.14401622269804193 1103 7 -0.006300355380560063 1103 8 -0.07288161771672463 1103 9 0.11420021404962455 1103 10 -0.10556561114876217 1103 11 0.07642432125724952 1103 12 0.19202660047218956 1103 13 0.30455604371062606 1103 14 0.24420767479606553 1103 15 0.20719511180193706 1103 16 0.29185740619364253 1103 17 0.14097690263241702 1103 18 -0.07295007416689714 1103 19 -0.16195941884016918 1103 20 -0.17879209470461177 1103 21 -0.13658456205269687 1103 22 -0.25540542910583497 1103 23 0.09028901159405933 1103 24 0.08595710834910619 1103 25 0.3648254457878579 1104 1 0.040295957164694585 1104 2 0.14799869608312832 1104 3 -0.04605129929085905 1104 4 0.12648220236487617 1104 5 -0.02137502411646608 1104 6 -0.011487412087042514 1104 7 0.1412161854823566 1104 8 0.31624024107244963 1104 9 0.07923919767960541 1104 10 0.24230504396260244 1104 11 -0.0903976586762738 1104 12 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9 -0.09038028481658955 1107 10 -0.09912570185171177 1107 11 -0.045344386225742994 1107 12 -0.05500287798585646 1107 13 -0.11712196082452664 1107 14 -0.1486267923241158 1107 15 -0.32251010361756727 1107 16 -0.20868681149342905 1107 17 -0.1532181087054525 1107 18 0.0882290641551009 1107 19 -0.03837665240025956 1107 20 -0.030356720554948424 1107 21 -0.08071154617967835 1107 22 0.029583233025682887 1107 23 -0.01905339203405987 1107 24 -0.01939436557379537 1107 25 -0.3271473433277876 1108 1 -0.13123406987598685 1108 2 -0.16952128727645288 1108 3 -0.14825615472722475 1108 4 -0.1826482367242619 1108 5 -0.35103855089466407 1108 6 -0.10782850104394726 1108 7 -0.18652081666702713 1108 8 -0.21800633427001515 1108 9 -0.12422458464787667 1108 10 -0.12461618148535791 1108 11 -0.24580232789843137 1108 12 -0.13245286593475925 1108 13 -0.19799928066063188 1108 14 -0.10105870362804836 1108 15 -0.01631583993957264 1108 16 -0.19787456757923394 1108 17 -0.21929186141694856 1108 18 -0.40011029472655935 1108 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17 0.42772677318431107 2213 18 0.46829030649980297 2213 19 0.4541867771710457 2213 20 0.4076747755080419 2213 21 0.2207145255613483 2213 22 0.23134176320940725 2213 23 0.12185649423015832 2213 24 0.3094157831576112 2213 25 0.26203025947186376 2214 1 0.1364853461623028 2214 2 0.1983900855156122 2214 3 0.1523885789435404 2214 4 0.09364897775082426 2214 5 0.17605920071502368 2214 6 0.304396475714654 2214 7 0.1785069008552611 2214 8 0.2562000149363328 2214 9 0.18081019137388665 2214 10 0.2719854092594005 2214 11 0.3312429153615568 2214 12 0.4060288680458868 2214 13 -0.019423548126113133 2214 14 0.3028811441838215 2214 15 0.28344227733481375 2214 16 0.3717893789827613 2214 17 0.28241681192946544 2214 18 0.3014830324090667 2214 19 0.29495668093307614 2214 20 0.2608337556355238 2214 21 0.45865353318539803 2214 22 0.08728164572409725 2214 23 0.5021370841564163 2214 24 0.26481198363255487 2214 25 0.11340821222776754 2215 1 0.10227899695595255 2215 2 0.031247228372755842 2215 3 0.22532508688004782 2215 4 0.2952362793381269 2215 5 0.2233607880166062 2215 6 0.22868219000037016 2215 7 0.02771325006161618 2215 8 0.14389842763474547 2215 9 0.1670800326437245 2215 10 -0.058490781216789856 2215 11 -0.18089994178129842 2215 12 0.11039394741096942 2215 13 0.08351295981039897 2215 14 -0.02799187168919559 2215 15 0.2190771979697388 2215 16 0.06401160120498162 2215 17 0.02106760558026366 2215 18 0.083149699075733 2215 19 0.07813128410767689 2215 20 0.1153575651593357 2215 21 0.18087575540761947 2215 22 0.13244037588282254 2215 23 -0.12159307457373814 2215 24 -0.2724102385896714 2215 25 -0.12397274015318516 2216 1 -0.14445428236711777 2216 2 -0.008266679269695487 2216 3 -0.2042329291429965 2216 4 -0.15652355503040383 2216 5 0.02130479737838442 2216 6 -0.08049415350490108 2216 7 -0.021346071209428885 2216 8 -0.16129555922275784 2216 9 -0.5470868590657005 2216 10 -0.6508249247040467 2216 11 -0.3772704521782728 2216 12 -0.34743303971659145 2216 13 -0.19711355922822094 2216 14 -0.6709876408853432 2216 15 -0.5383051046677636 2216 16 -0.3738747972107177 2216 17 -0.38372850568087813 2216 18 -0.33913493500474345 2216 19 -0.557490283990262 2216 20 -0.24158545401430587 2216 21 -0.10978947098539749 2216 22 -0.21538211038956945 2216 23 -0.5009647551930598 2216 24 -0.5052880853805561 2216 25 -0.2913649446720399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 53.0 10 119.0 11 171.0 12 172.0 13 297.0 14 493.0 15 800.0 16 1126.0 17 1305.0 18 1430.0 19 1433.0 20 1416.0 21 1475.0 22 1299.0 23 1254.0 24 1382.0 25 1353.0 26 1336.0 27 1439.0 28 1678.0 29 1975.0 30 2485.0 31 3375.0 32 4556.0 33 6448.0 34 9467.0 35 14613.0 36 24765.0 37 49636.0 38 161018.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.166250938740475 18.717479347709475 12.017554446947752 23.098715266602294 2 12.11809350130403 24.938821210434924 33.50485742827834 29.438227859982703 3 22.797427329429865 31.26384620283074 27.386071515951834 18.552654951787563 4 13.90666108085463 22.91813992459154 27.877335567658147 35.29786342689569 5 10.348051948051948 39.85152911604525 34.96908253037285 14.831336405529955 6 31.864437457682794 29.170549235441207 18.379577799974527 20.585435506901476 7 19.683954754922496 35.92760787599497 23.412484289903645 20.975953079178886 8 28.861332216170926 31.64574780058651 22.08596564725597 17.406954335986594 9 27.731545873481355 16.254377880184332 20.477251780477587 35.536824465856725 10 19.485546711353162 31.76170925848345 29.475994972769165 19.27674905739422 11 25.153917050691245 27.340092165898618 17.453540008378717 30.05245077503142 12 25.673062421449515 33.558106409719315 26.924507750314202 13.844323418516966 13 31.86996229576875 20.18667783829074 22.8058651026393 25.137494763301216 14 22.360452450775032 29.321156263091748 25.96196062002514 22.356430666108086 15 37.186761625471306 18.61114369501466 23.608211143695016 20.593883535819018 16 15.475157100963552 26.912107247591116 37.04465856723921 20.568077084206116 17 25.85605362379556 29.46392961876833 27.91118558860494 16.76883116883117 18 25.73472978634269 20.446418098031003 33.28127356514453 20.53757855048178 19 22.089987431922918 30.711688311688313 34.28806032677 12.910263929618768 20 23.52442396313364 22.962379555927942 31.974528697109346 21.538667783829073 21 17.624130708001676 28.174612484289902 35.38299120234604 18.81826560536238 22 21.700879765395893 26.530708001675745 24.53891914537076 27.229493087557604 23 15.661164641809803 26.530372852953498 33.23435274403017 24.574109761206536 24 20.60728948470884 34.697947214076244 27.10917469627147 17.585588604943446 25 20.983661499790532 22.313866778382906 25.53833263510683 31.164139086719732 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1963.0 1 1963.0 2 1637.0 3 1311.0 4 1311.0 5 1311.0 6 2475.0 7 3639.0 8 3639.0 9 3639.0 10 3320.0 11 3001.0 12 3001.0 13 3001.0 14 2320.0 15 1639.0 16 1639.0 17 1639.0 18 2480.0 19 3321.0 20 3321.0 21 3321.0 22 4980.5 23 6640.0 24 6640.0 25 6640.0 26 8588.5 27 10537.0 28 10537.0 29 10537.0 30 11416.0 31 12295.0 32 12295.0 33 12295.0 34 12730.5 35 13166.0 36 13166.0 37 13166.0 38 11827.0 39 10488.0 40 10488.0 41 10488.0 42 14130.5 43 17773.0 44 17773.0 45 17773.0 46 59930.5 47 102088.0 48 102088.0 49 102088.0 50 85697.0 51 69306.0 52 69306.0 53 69306.0 54 46322.5 55 23339.0 56 23339.0 57 23339.0 58 15551.0 59 7763.0 60 7763.0 61 7763.0 62 6518.0 63 5273.0 64 5273.0 65 5273.0 66 3986.5 67 2700.0 68 2700.0 69 2700.0 70 1970.0 71 1240.0 72 1240.0 73 1240.0 74 919.5 75 599.0 76 599.0 77 599.0 78 417.0 79 235.0 80 235.0 81 235.0 82 140.5 83 46.0 84 46.0 85 46.0 86 28.0 87 10.0 88 10.0 89 10.0 90 6.0 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03452031839128613 2 0.023125261834939254 3 0.0026811897779639715 4 0.0 5 0.0 6 0.011059907834101382 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 298375.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.4184331797235 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12248284522582 17.28965228320067 2 12.89710125427935 5.782656053623795 3 4.389230240241587 2.9519899455383327 4 1.9599049199443872 1.7575198994553831 5 0.9956496389648831 1.1160452450775031 6 0.5815431074434528 0.7822371177209887 7 0.3707524181130496 0.5818181818181818 8 0.2421850473157824 0.43435274403016333 9 0.1913560867680256 0.3860913280268119 >10 0.9522955255565023 4.201089233347298 >50 0.10614282937913919 1.6770842061164641 >100 0.1076377988070144 5.305404273146209 >500 0.022424541418128 3.3501466275659824 >1k 0.044849082836256 23.137997486384585 >5k 0.0134547248508768 18.6195224130708 >10k+ 0.0029899388557504 12.626392961876833 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 19180 6.428152492668622 No Hit GTACATGGGAAGCAGTGGTATCAAC 18494 6.198240469208211 No Hit GAGTACATGGGAAGCAGTGGTATCA 9019 3.0227063259321323 No Hit CATGTACTCTGCGTTGATACCACTG 7473 2.504566401340595 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6151 2.0614997905320487 No Hit GCGTTGATACCACTGCTTCCCATGT 6055 2.029325513196481 No Hit GTATCAACGCAGAGTACTTTTTTTT 5837 1.9562630917469626 No Hit TATCAACGCAGAGTACATGGGAAGC 5438 1.8225387515710096 No Hit GGTATCAACGCAGAGTACATGGGAA 5303 1.7772936740678678 No Hit GTATCAACGCAGAGTACATGGGAAG 5197 1.7417679095098448 No Hit ACTCTGCGTTGATACCACTGCTTCC 5083 1.7035609551738586 No Hit GGTATCAACGCAGAGTACTTTTTTT 4824 1.6167574361122747 No Hit TATCAACGCAGAGTACTTTTTTTTT 4007 1.3429409300377042 No Hit GCTTCCCATGTACTCTGCGTTGATA 3593 1.2041893590280688 No Hit GTGGTATCAACGCAGAGTACATGGG 3434 1.1509007121910348 No Hit GTACTCTGCGTTGATACCACTGCTT 3027 1.0144951822371178 No Hit GATACCACTGCTTCCCATGTACTCT 2836 0.9504817762882278 No Hit ATACCACTGCTTCCCATGTACTCTG 2758 0.9243401759530793 No Hit CATGGGAAGCAGTGGTATCAACGCA 2584 0.8660242982823628 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2572 0.8620025136154168 No Hit CAGTGGTATCAACGCAGAGTACATG 2534 0.8492668621700881 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2452 0.8217846669459572 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2450 0.8211143695014663 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2367 0.7932970255550901 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2275 0.7624633431085044 No Hit ACATGGGAAGCAGTGGTATCAACGC 2246 0.752744030163385 No Hit GCAGTGGTATCAACGCAGAGTACAT 2215 0.7423544197737746 No Hit TACCACTGCTTCCCATGTACTCTGC 2139 0.7168831168831169 No Hit CTTCCCATGTACTCTGCGTTGATAC 2011 0.6739840804356934 No Hit GTTGATACCACTGCTTCCCATGTAC 2006 0.6723083368244658 No Hit CCACTGCTTCCCATGTACTCTGCGT 1887 0.6324256388772518 No Hit CCATGTACTCTGCGTTGATACCACT 1794 0.6012568077084206 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1751 0.5868454126518642 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1749 0.5861751152073733 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1643 0.5506493506493506 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1593 0.5338919145370759 No Hit TTCCCATGTACTCTGCGTTGATACC 1426 0.47792207792207786 No Hit TGATACCACTGCTTCCCATGTACTC 1409 0.47222454964390453 No Hit ATCAACGCAGAGTACATGGGAAGCA 1266 0.42429828236279843 No Hit GTACATGGTAAGCAGTGGTATCAAC 1154 0.3867616254713029 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1036 0.3472140762463343 No Hit GGAAGCAGTGGTATCAACGCAGAGT 923 0.3093422706325932 No Hit ACCATGTACTCTGCGTTGATACCAC 920 0.30833682446585675 No Hit GTGGTATCAACGCAGAGTACTTTTT 898 0.3009635525764558 No Hit TCAACGCAGAGTACATGGGAAGCAG 720 0.24130708001675744 No Hit TGGGAAGCAGTGGTATCAACGCAGA 684 0.22924172601591955 No Hit ATCAACGCAGAGTACTTTTTTTTTT 678 0.22723083368244656 No Hit CTGCGTTGATACCACTGCTTCCCAT 669 0.2242144951822371 No Hit CTCTGCGTTGATACCACTGCTTCCC 658 0.22052785923753665 No Hit AAAAAGTACTCTGCGTTGATACCAC 622 0.2084625052366988 No Hit CCCCATGTACTCTGCGTTGATACCA 599 0.20075408462505237 No Hit TCCATGTACTCTGCGTTGATACCAC 559 0.1873481357352325 No Hit GGTAAGCAGTGGTATCAACGCAGAG 530 0.1776288227901131 No Hit AACGCAGAGTACATGGGAAGCAGTG 526 0.17628822790113113 No Hit TCCCATGTACTCTGCGTTGATACCA 506 0.16958525345622122 No Hit AGTGGTATCAACGCAGAGTACATGG 504 0.1689149560117302 No Hit AAAGTACTCTGCGTTGATACCACTG 481 0.16120653540008378 No Hit GAGTACATGGTAAGCAGTGGTATCA 461 0.15450356095517387 No Hit GTACATGGAAAGCAGTGGTATCAAC 438 0.14679514034352745 No Hit ATGTACTCTGCGTTGATACCACTGC 434 0.14545454545454545 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 434 0.14545454545454545 No Hit ACTGCTTCCCATGTACTCTGCGTTG 408 0.13674067867616255 No Hit GCTTACCATGTACTCTGCGTTGATA 405 0.13573523250942607 No Hit TTGATACCACTGCTTCCCATGTACT 402 0.13472978634268956 No Hit CAACGCAGAGTACATGGGAAGCAGT 374 0.12534562211981565 No Hit GTACATGGGTAAGCAGTGGTATCAA 368 0.12333472978634269 No Hit AACGCAGAGTACTTTTTTTTTTTTT 355 0.11897779639715124 No Hit CAACGCAGAGTACTTTTTTTTTTTT 344 0.11529116045245076 No Hit GAGTACATGGAAAGCAGTGGTATCA 342 0.11462086300795978 No Hit CGTTGATACCACTGCTTCCCATGTA 328 0.10992878089652283 No Hit CGCAGAGTACATGGGAAGCAGTGGT 325 0.10892333472978634 No Hit ACTCTGCGTTGATACCACTGCTTAC 324 0.10858818600754083 No Hit GCGTTGATACCACTGCTTACCATGT 320 0.10724759111855886 No Hit ACGCAGAGTACATGGTAAGCAGTGG 313 0.1049015500628404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 3.3514872224549644E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATAA 25 0.0060123224 19.004694 2 GTACAAA 40 0.005245567 14.258302 1 GAACAAA 115 2.5465852E-11 14.051659 1 ATGGCAA 55 1.9504948E-4 13.819279 5 AAAAAGT 140 0.0 13.579334 1 AAAAGTA 140 0.0 13.574781 2 CATGGCA 70 1.08440916E-4 12.215255 4 AGTACTC 165 0.0 12.091869 5 TGGCAAG 65 7.9848064E-4 11.693235 6 AAGTACT 175 1.8189894E-12 11.400906 4 ACATGGC 85 5.2850028E-5 11.177358 3 AAAGTAC 170 1.0913936E-11 11.177358 3 GGCAAGC 70 0.0014863863 10.856185 7 CAAGCAG 70 0.0014863863 10.856185 9 GCAAGCA 80 0.0045006955 9.499162 8 ACACAAA 95 0.0018048893 9.005243 1 CACAAAA 95 0.0018104092 9.002224 2 ACAAAAA 455 0.0 8.143503 3 CCCCATG 270 1.2732926E-10 8.097307 1 AATCAAC 160 5.738122E-5 7.718069 19 >>END_MODULE