FastQCFastQC Report
Wed 25 May 2016
SRR1294902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169206
Sequences flagged as poor quality0
Sequence length25
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC259822.222191812221285No Hit
CCCATGTACTCTGCGTTGATACCAC237892.0346286283169945No Hit
GTATCAACGCAGAGTACTTTTTTTT132961.13718198503942No Hit
GAGTACATGGGAAGCAGTGGTATCA115190.9851985022314288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA88000.7526475231909519No Hit
GGTATCAACGCAGAGTACTTTTTTT87900.7517922419145984No Hit
CATGTACTCTGCGTTGATACCACTG85050.7274167255385279No Hit
GCGTTGATACCACTGCTTCCCATGT84100.719291553413171No Hit
GTATCAACGCAGAGTACATGGGAAG78330.6699418237675825No Hit
TATCAACGCAGAGTACTTTTTTTTT77330.6613890110040489No Hit
GGTATCAACGCAGAGTACATGGGAA71870.6146906533151557No Hit
TATCAACGCAGAGTACATGGGAAGC68920.5894598556627318No Hit
ACGCAGAGTACATGGGAAGCAGTGG67580.5779990865595969No Hit
ACTCTGCGTTGATACCACTGCTTCC58580.501023771687795No Hit
GTGGTATCAACGCAGAGTACATGGG52390.44808186068152234No Hit
GCTTCCCATGTACTCTGCGTTGATA42640.36469193623707025No Hit
GTACTCTGCGTTGATACCACTGCTT35930.3073025625937602No Hit
ATACCACTGCTTCCCATGTACTCTG33220.2841244400045843No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33110.2831836306005956No Hit
GGGAAGCAGTGGTATCAACGCAGAG30730.2628279362233858No Hit
GCAGAGTACATGGGAAGCAGTGGTA30240.25863705796925435No Hit
CAGTGGTATCAACGCAGAGTACATG30210.2583804735863483No Hit
CTGCTTCCCATGTACTCTGCGTTGA28380.24272882622908193No Hit
GATACCACTGCTTCCCATGTACTCT28300.24204460120799925No Hit
GCAGTGGTATCAACGCAGAGTACAT27920.23879453235785653No Hit
CATGGGAAGCAGTGGTATCAACGCA27540.2355444635077138No Hit
TACCACTGCTTCCCATGTACTCTGC25610.21903753487409403No Hit
GGTATCAACGCAGAGTACATGGGGA24840.21245186904617322No Hit
GTTGATACCACTGCTTCCCATGTAC24270.20757676577095907No Hit
ACATGGGAAGCAGTGGTATCAACGC23770.20330035938919233No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21450.1834578337777945No Hit
CTTCCCATGTACTCTGCGTTGATAC20850.17832614611967437No Hit
CCACTGCTTCCCATGTACTCTGCGT20830.1781550898644037No Hit
AAGCAGTGGTATCAACGCAGAGTAC19710.16857593956924613No Hit
CCATGTACTCTGCGTTGATACCACT19150.16378636442166736No Hit
ATGGGAAGCAGTGGTATCAACGCAG19000.16250344250713733No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17830.15249665157380307No Hit
ATCAACGCAGAGTACATGGGAAGCA15820.13530549791910065No Hit
TGATACCACTGCTTCCCATGTACTC15710.13436468851511196No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15610.13350940723875862No Hit
TTCCCATGTACTCTGCGTTGATACC15340.13120014779260455No Hit
GGTATCAACGCAGAGTACATGGGGG15170.12974616962280386No Hit
GTATCAACGCAGAGTACATGGGGAG14260.12196311000798833No Hit
GGTATCAACGCAGAGTACATGGGCA14030.11999596307237562No Hit
GTGGTATCAACGCAGAGTACTTTTT12920.11050234090485339No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12800.10947600337322937No Hit
ATCAACGCAGAGTACTTTTTTTTTT11760.10058107809915447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAA851.6832928E-814.55011
CGCAAAA1155.316906E-912.3914172
GTCCTGG701.0760623E-412.2316771
GTAAAAA5200.011.891911
GTACAAA3200.011.5946111
CTAGACT951.35914615E-511.0000994
ACTTACC750.002650294210.1316898
GGGAACC750.002650294210.1316898
AGTACCA856.5897853E-410.0589145
GAACAAA6650.09.8711791
CTATACA900.00111298539.5000854
TAAGTAG900.00111298539.5000855
TTAAAGC800.0045136969.5000853
ACCTATG800.00452007739.4984587
GACTGTC800.00452007739.4984587
TCCTGGA1252.7508497E-59.1200822
GCTATAA1054.4259155E-49.0605021
TTCTAGG1054.488923E-49.04772
CCTAGAC950.00182260299.0000813
GTACAAG850.0073462028.9539081
Produced by FastQC (version 0.11.5)