FastQCFastQC Report
Wed 25 May 2016
SRR1294902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169206
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT341662.9221540087888704No Hit
GTACATGGGAAGCAGTGGTATCAAC263912.2571728164241374No Hit
CCCATGTACTCTGCGTTGATACCAC260642.2292051186873825No Hit
GGTATCAACGCAGAGTACTTTTTTT232691.9901540019466202No Hit
TATCAACGCAGAGTACTTTTTTTTT205731.7595701698417558No Hit
GAGTACATGGGAAGCAGTGGTATCA119011.0178702469881271No Hit
ACGCAGAGTACTTTTTTTTTTTTTT100160.8566497263955197No Hit
CATGTACTCTGCGTTGATACCACTG92170.7883127524148867No Hit
GCGTTGATACCACTGCTTCCCATGT83120.7109097969049082No Hit
GTATCAACGCAGAGTACATGGGAAG77240.6606192578553309No Hit
ACGCAGAGTACATGGGAAGCAGTGG74870.6403490916057564No Hit
TATCAACGCAGAGTACATGGGAAGC72900.6235000504615953No Hit
GTACTTTTTTTTTTTTTTTTTTTTT72470.6198223409732759No Hit
GGTATCAACGCAGAGTACATGGGAA72370.6189670596969226No Hit
ACTCTGCGTTGATACCACTGCTTCC62580.5352350227419291No Hit
GAGTACTTTTTTTTTTTTTTTTTTT56450.4828062805014685No Hit
GTGGTATCAACGCAGAGTACATGGG51470.4402132729390715No Hit
GCTTCCCATGTACTCTGCGTTGATA43890.3753829521914872No Hit
GCAGAGTACTTTTTTTTTTTTTTTT41320.353402223389206No Hit
ATACCACTGCTTCCCATGTACTCTG35680.30516435940287684No Hit
GTACTCTGCGTTGATACCACTGCTT34340.29370359029974186No Hit
CAGTGGTATCAACGCAGAGTACATG33560.2870323963441857No Hit
ATCAACGCAGAGTACTTTTTTTTTT32890.28130201179261827No Hit
GGGAAGCAGTGGTATCAACGCAGAG32870.28113095553734757No Hit
CATGGGAAGCAGTGGTATCAACGCA31440.26890043328549457No Hit
GTGGTATCAACGCAGAGTACTTTTT31010.26522272379717515No Hit
GATACCACTGCTTCCCATGTACTCT29710.25410406720458156No Hit
GCAGAGTACATGGGAAGCAGTGGTA28840.2466631201003074No Hit
CTGCTTCCCATGTACTCTGCGTTGA28170.24093273554873992No Hit
ACATGGGAAGCAGTGGTATCAACGC27690.23682738542224382No Hit
GCAGTGGTATCAACGCAGAGTACAT27610.23614316040116112No Hit
TACCACTGCTTCCCATGTACTCTGC26440.22613636946782686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24950.2133926784501619No Hit
GGTATCAACGCAGAGTACATGGGGA24510.20962944083420715No Hit
GTTGATACCACTGCTTCCCATGTAC23460.20064898743249693No Hit
CCACTGCTTCCCATGTACTCTGCGT23290.19919500926269623No Hit
CTTCCCATGTACTCTGCGTTGATAC22720.1943199059874821No Hit
CCATGTACTCTGCGTTGATACCACT21300.18217491186326445No Hit
AAGCAGTGGTATCAACGCAGAGTAC19900.1702009739943175No Hit
ATGGGAAGCAGTGGTATCAACGCAG19630.16789171454816346No Hit
TGATACCACTGCTTCCCATGTACTC16820.14385831068263422No Hit
ATCAACGCAGAGTACATGGGAAGCA16750.14325961378918683No Hit
GGTATCAACGCAGAGTACATGGGGG16000.1368450042165367No Hit
TTCCCATGTACTCTGCGTTGATACC15940.13633183545072466No Hit
GTATCAACGCAGAGTACATGGGGAG15810.13521996979146533No Hit
GGTATCAACGCAGAGTACATGGGCA15260.13051592277152185No Hit
CAACGCAGAGTACTTTTTTTTTTTT14540.12435789758177772No Hit
AACGCAGAGTACTTTTTTTTTTTTT12240.10468642822565057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC307.724323E-418.99884416
CGGTATC555.886541E-717.2783282
CGAGACA350.002170648716.28472317
GCGGTAT601.4546022E-615.8452461
GACTGCT456.765867E-414.7762477
CAACGAG400.005281695614.24852514
ACTGTTC1001.9304462E-611.398828
GAACAAA4700.011.1253851
TAAAGGT951.3589124E-511.0002724
GTCTCGA700.001491679910.856482516
GTATTAC1002.3771627E-510.4578631
ACAGTGC750.002649007410.1322848
GGCAAGC856.596619E-410.0577827
CCTATTC951.6426216E-410.0002473
TCTTAGC1002.7445742E-49.5030792
CTTATTC1201.698195E-59.5002363
TAGCTCT1054.4882263E-49.0478434
GTCTCGC950.0018103229.0067711
CGCTCTG950.00182234489.0002225
AAAGGTG950.00182234489.0002225
Produced by FastQC (version 0.11.5)