FastQCFastQC Report
Wed 25 May 2016
SRR1294901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085540
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC124011.1423807505941743No Hit
CCCATGTACTCTGCGTTGATACCAC120381.108941172135527No Hit
GAGTACATGGGAAGCAGTGGTATCA57940.5337435746264532No Hit
CATGTACTCTGCGTTGATACCACTG51690.4761685428450357No Hit
GCGTTGATACCACTGCTTCCCATGT37730.3475689518580614No Hit
ACGCAGAGTACATGGGAAGCAGTGG32350.2980083645006172No Hit
GTATCAACGCAGAGTACATGGGAAG30000.2763601525508042No Hit
TATCAACGCAGAGTACATGGGAAGC29090.2679772279234298No Hit
ACTCTGCGTTGATACCACTGCTTCC27210.2506586583635794No Hit
GGTATCAACGCAGAGTACATGGGAA26670.24568417561766495No Hit
GTATCAACGCAGAGTACTTTTTTTT25400.2339849291596809No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23940.22053540173554176No Hit
GTGGTATCAACGCAGAGTACATGGG20900.19253090627706027No Hit
GCTTCCCATGTACTCTGCGTTGATA16990.15651196639460543No Hit
GTACTCTGCGTTGATACCACTGCTT16090.14822116181808132No Hit
GGTATCAACGCAGAGTACTTTTTTT16000.14739208136042892No Hit
ATACCACTGCTTCCCATGTACTCTG15770.14527332019087275No Hit
CAGTGGTATCAACGCAGAGTACATG15490.14269395876706525No Hit
TATCAACGCAGAGTACTTTTTTTTT15370.14158851815686202No Hit
GGGAAGCAGTGGTATCAACGCAGAG14310.13182379276673362No Hit
GCAGAGTACATGGGAAGCAGTGGTA14000.12896807119037532No Hit
CTGCTTCCCATGTACTCTGCGTTGA13200.12159846712235386No Hit
GCAGTGGTATCAACGCAGAGTACAT12910.11892698564769608No Hit
CATGGGAAGCAGTGGTATCAACGCA12740.11736094478324152No Hit
GATACCACTGCTTCCCATGTACTCT12380.11404462295263187No Hit
TACCACTGCTTCCCATGTACTCTGC12240.1127549422407281No Hit
GTTGATACCACTGCTTCCCATGTAC12210.1124785820881773No Hit
CCACTGCTTCCCATGTACTCTGCGT10920.10059509552849273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA250.006037355418.99649611
TATTCCG350.00216896316.2864655
CGCAAGA550.00306530112.0920232
CCTATAG550.00306530112.0920233
AGTGCGG550.003066359812.0914675
TAACGCG550.003070597812.08923612
TGTCCCA550.003072718612.08812210
CGAACTC658.01999E-411.69230815
TTACACA1001.9254821E-611.40105154
TGTACCG752.0718265E-411.4005255
ACCGATC600.005873718311.08333419
TGCGGTA600.00588178611.081297
GTGCGGT600.00588178611.081296
ATACCGT600.00588178611.081296
AGGACCG1303.2581738E-810.9620455
GGACCGC1303.2692697E-810.9595186
GCGCAAG700.001477066810.8691691
TGGATTG803.7670904E-410.6879925
AACGTCT803.775123E-410.685536
GTCTTAG1002.3674344E-510.4615751
Produced by FastQC (version 0.11.5)