FastQCFastQC Report
Wed 25 May 2016
SRR1294901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085540
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC132261.2183797925456454No Hit
GTACATGGGAAGCAGTGGTATCAAC117411.0815815170329974No Hit
GTATCAACGCAGAGTACTTTTTTTT73270.6749636125799142No Hit
GAGTACATGGGAAGCAGTGGTATCA55840.514398363947897No Hit
CATGTACTCTGCGTTGATACCACTG54170.4990143154559022No Hit
GGTATCAACGCAGAGTACTTTTTTT45670.42071227223317426No Hit
TATCAACGCAGAGTACTTTTTTTTT43900.40440702323267685No Hit
GCGTTGATACCACTGCTTCCCATGT39240.3614790795364519No Hit
ACGCAGAGTACATGGGAAGCAGTGG34120.3143136135011147No Hit
ACTCTGCGTTGATACCACTGCTTCC29960.2759916723474031No Hit
TATCAACGCAGAGTACATGGGAAGC28910.26631906700812497No Hit
GTATCAACGCAGAGTACATGGGAAG28600.2634633454317667No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25760.23730125099029054No Hit
GGTATCAACGCAGAGTACATGGGAA25690.23665641063433865No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22710.20920463548095877No Hit
GTGGTATCAACGCAGAGTACATGGG19740.18184498037842917No Hit
GCTTCCCATGTACTCTGCGTTGATA18560.17097481437809753No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17600.1621312894964718No Hit
ATACCACTGCTTCCCATGTACTCTG16710.15393260497079794No Hit
CAGTGGTATCAACGCAGAGTACATG15990.14729996130957865No Hit
GTACTCTGCGTTGATACCACTGCTT15910.1465630009027765No Hit
CTGCTTCCCATGTACTCTGCGTTGA14020.12915231129207583No Hit
GCAGAGTACATGGGAAGCAGTGGTA13800.12712567017336993No Hit
GGGAAGCAGTGGTATCAACGCAGAG13730.12648082981741804No Hit
CATGGGAAGCAGTGGTATCAACGCA13610.12537538920721483No Hit
GATACCACTGCTTCCCATGTACTCT13280.12233542752915601No Hit
TACCACTGCTTCCCATGTACTCTGC13240.12196694732575493No Hit
GCAGTGGTATCAACGCAGAGTACAT13110.12076938666470145No Hit
CCACTGCTTCCCATGTACTCTGCGT12280.1131234224441292No Hit
GTTGATACCACTGCTTCCCATGTAC12230.11266282218987785No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10930.100687215579343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCC504.5636443E-617.09846514
CGCAAGA801.2842611E-714.2526612
AACGCCG602.5645653E-514.2513475
GTCTAGA551.9500859E-413.8258581
GTTAGAC551.9569593E-413.8201263
CGGTAGC707.2644507E-613.5702110
CGACGGG500.001500052713.29880714
GGTAGCG655.4541226E-513.15266611
AAGACGG751.4761741E-512.6678655
TAACGCC604.086998E-412.6678644
GCGGCCC751.4789737E-512.66553115
CAGTGCG604.0933565E-412.665539
CTAGAAC1155.304173E-912.3930463
ACGGTAT550.003069483212.0898249
GCGCAAG951.0318563E-612.0066661
AGACAGC1209.982614E-911.8761226
CTAGACT658.0136413E-411.6934144
TCGCCGG658.0260396E-411.69125817
CTCGTGA658.0260396E-411.69125817
CCGACGG658.0260396E-411.69125813
Produced by FastQC (version 0.11.5)