FastQCFastQC Report
Wed 25 May 2016
SRR1294900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4191367
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.011.7618451
GTATCAA31450.010.283581
ACTGTCG1054.1176998E-59.9504048
CTAGACT4000.09.5020854
AACCGTG1304.253854E-69.4981147
GTCCTAA3150.09.3613211
TGAACCG1252.7544156E-59.12058455
GTCTTAC4500.08.8781581
GTACTAA3350.08.8024371
ACCGTGT3250.08.7674898
GTACTAG3150.08.7573651
TATACTG8350.08.6472625
CTAGGAC3550.08.565263
TATCCCG1000.00290484248.5505495
GAACCGT1000.00291175868.5483036
CCTACAC5300.08.4263763
TAGACTG4750.08.4005385
TATACCG1252.7432843E-48.3605365
CTAGACA4800.08.3143234
GTCCAAG6350.08.2390091
Produced by FastQC (version 0.11.5)