FastQCFastQC Report
Wed 25 May 2016
SRR1294900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4191367
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106180.2533302380822295No Hit
GGTATCAACGCAGAGTACTTTTTTT62530.1491876039487833No Hit
TATCAACGCAGAGTACTTTTTTTTT59490.14193460033444938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.013.372361
CGACGTC600.005882684611.08226816
GTATCAA43150.010.9720561
TAAGACG909.517304E-510.5561784
CCCGACG750.002651286310.132359514
GTACCGT1151.0268965E-59.9135116
GAACCGT1002.75604E-49.5004476
TTCGCAA800.0045217299.49908714
CTAGGAC4200.09.2744683
GTCCTAA3900.09.019381
TTATACT6900.08.9498034
ACACCGG850.007436288.9415976
TATTCCG1701.6859667E-78.9415975
GCGTGCG850.0074450178.9403179
CCGTGCT2356.548362E-118.8927639
TAGACAG5950.08.7819265
TGTACCG1752.6266753E-78.6861245
GTCTTAG3650.08.5952911
TCAAGAC6750.08.5857943
GTGCTAG4550.08.56666851
Produced by FastQC (version 0.11.5)