FastQCFastQC Report
Wed 25 May 2016
SRR1294899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036398
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37190.12248064976989183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG658.0232567E-411.6930075
TAACGCT600.005870911.08509354
CCGTGTT1356.4694177E-79.8495199
CTGGACG800.0045116279.5015094
TAGACAG5400.09.5005695
TACACCG900.00111354749.5005685
CCGTTCA800.0045263759.497759
TAGACTG5100.09.1279975
ATTATAC6000.09.0262843
ATCCGAA950.00182706488.998610512
GTTACCC1804.2127795E-88.9734983
CGTGTTA850.0074485998.93964910
CCGTCCA850.0074526288.9390599
TAGTACT3550.08.8323894
GTATCAA30800.08.7409471
ACCGTGT1651.0540425E-68.634468
GCCCTAT2002.4814653E-88.5618051
GTGTTAG4700.08.5010831
GTGTAAG3700.08.4846711
GTTACAC2252.4429028E-98.4456453
Produced by FastQC (version 0.11.5)