FastQCFastQC Report
Wed 25 May 2016
SRR1294899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036398
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91310.30071815354904063No Hit
GGTATCAACGCAGAGTACTTTTTTT51310.1689831174964547No Hit
TATCAACGCAGAGTACTTTTTTTTT51140.1684232435932312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGC604.0928563E-412.6670953
CGGGCGG550.00307147812.08992517
GCACCGT951.3610059E-511.0001926
AGCACCG750.002648433710.133515
CCCTTCG750.002650605510.13250813
GTCTCGC1606.1118044E-910.1005781
ATAGCGA856.601747E-410.0580038
TCTCGCT1804.1654857E-99.5028262
CGAGGCG800.00452058169.49922612
CCGTGTT1451.7094408E-69.1716669
GCGTGCA1054.4982528E-49.0468829
ACCGTGT2054.150934E-98.8041618
AGGCGTG4400.08.635667
GTATCAA39850.08.5402781
GTTACAC2451.5643309E-108.53093
GTATTAA5750.08.43178751
TTAGACA3950.08.4178684
GTACTAA3400.08.3880231
CTCGCTC2053.8620783E-88.3417453
GTACTAG2409.222276E-108.3181241
Produced by FastQC (version 0.11.5)