FastQCFastQC Report
Wed 25 May 2016
SRR1294898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666040
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1629496.112023825599016No Hit
CCCATGTACTCTGCGTTGATACCAC1565185.870804639090186No Hit
GAGTACATGGGAAGCAGTGGTATCA699972.6255044935559857No Hit
CATGTACTCTGCGTTGATACCACTG559742.099518386820903No Hit
GCGTTGATACCACTGCTTCCCATGT500781.8783664161077855No Hit
GTATCAACGCAGAGTACATGGGAAG438161.6434862192615265No Hit
TATCAACGCAGAGTACATGGGAAGC380371.4267227798532656No Hit
ACGCAGAGTACATGGGAAGCAGTGG370701.3904517561626983No Hit
ACTCTGCGTTGATACCACTGCTTCC331821.2446174851090006No Hit
GGTATCAACGCAGAGTACATGGGAA304021.140342980600441No Hit
GCTTCCCATGTACTCTGCGTTGATA288221.0810790535775907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA232780.8731301855936145No Hit
GTACTCTGCGTTGATACCACTGCTT212010.7952243777287662No Hit
ATACCACTGCTTCCCATGTACTCTG195610.7337099218316304No Hit
GTGGTATCAACGCAGAGTACATGGG186130.6981515656179202No Hit
CAGTGGTATCAACGCAGAGTACATG179130.6718953954179232No Hit
GGGAAGCAGTGGTATCAACGCAGAG177320.6651062999804954No Hit
CTGCTTCCCATGTACTCTGCGTTGA167530.628385170515071No Hit
CATGGGAAGCAGTGGTATCAACGCA161840.6070426550239306No Hit
GCAGTGGTATCAACGCAGAGTACAT161500.6057673553285022No Hit
TACCACTGCTTCCCATGTACTCTGC156500.58701294804279No Hit
GCAGAGTACATGGGAAGCAGTGGTA145580.5460533225307947No Hit
CCATGTACTCTGCGTTGATACCACT143880.5396768240536526No Hit
GATACCACTGCTTCCCATGTACTCT139800.5243732277085116No Hit
GTTGATACCACTGCTTCCCATGTAC129510.48577665751451593No Hit
ACATGGGAAGCAGTGGTATCAACGC124910.4685226028116608No Hit
CCACTGCTTCCCATGTACTCTGCGT124490.4669472325996609No Hit
CTTCCCATGTACTCTGCGTTGATAC121210.45464434142023374No Hit
ATGGGAAGCAGTGGTATCAACGCAG112450.42178661985566607No Hit
AAGCAGTGGTATCAACGCAGAGTAC111090.4166854210739524No Hit
CCCCATGTACTCTGCGTTGATACCA100030.3752006721579571No Hit
ATCAACGCAGAGTACATGGGAAGCA88890.3334158527253905No Hit
TTCCCATGTACTCTGCGTTGATACC86680.3251264047051057No Hit
TGATACCACTGCTTCCCATGTACTC83730.31406130440653557No Hit
TCCATGTACTCTGCGTTGATACCAC69510.2607237700859702No Hit
GTACATGGAAAGCAGTGGTATCAAC60820.2281286102234025No Hit
GGAAGCAGTGGTATCAACGCAGAGT60120.2255029932034028No Hit
GTACATGGGGAGCAGTGGTATCAAC49000.18379319139997902No Hit
TGGGAAGCAGTGGTATCAACGCAGA48180.1807174686051222No Hit
AAACAAAAAAAAAAAAAAAAAAAAA47470.1780543427705511No Hit
TCCCATGTACTCTGCGTTGATACCA46440.17419093486969436No Hit
AAAAAGTACTCTGCGTTGATACCAC45080.16908973608798067No Hit
GCTTTCCATGTACTCTGCGTTGATA44220.16586397803483818No Hit
CTGCGTTGATACCACTGCTTCCCAT42570.1596750236305532No Hit
GTATCAACGCAGAGTACTTTTTTTT40490.15187319019969692No Hit
GAGTACATGGAAAGCAGTGGTATCA40070.15029781998769712No Hit
CTCTGCGTTGATACCACTGCTTCCC38940.14605932394112617No Hit
GTACATGGTAAGCAGTGGTATCAAC38140.1430586187754122No Hit
GTACATGGGAAAGCAGTGGTATCAA35350.13259365950998486No Hit
TCAACGCAGAGTACATGGGAAGCAG34800.1305306747085565No Hit
ACCATGTACTCTGCGTTGATACCAC33550.12584207288712848No Hit
AAAGTACTCTGCGTTGATACCACTG31310.11744009842312943No Hit
GCGTTGATACCACTGCTTTCCATGT31200.11702750146284377No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA29910.11218886438313003No Hit
ATGGAAAGCAGTGGTATCAACGCAG29580.11095107350227304No Hit
AGTGGTATCAACGCAGAGTACATGG27330.10251159022370256No Hit
TTGATACCACTGCTTCCCATGTACT27040.10142383460113127No Hit
GAGTAAGCAGTGGTATCAACGCAGA26900.10089871119713133No Hit
ACTCTGCGTTGATACCACTGCTTTC26780.10044860542227424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT400.00527117814.253764
GTAGCAC707.2516395E-613.5742453
ATAGACC604.08657E-412.6692953
TCTGTCG604.098886E-412.6647788
GTCTTAC1104.8816764E-711.2433271
GTACAAA2800.011.2124391
AGCGGTA1502.1409505E-910.7650614
TAGACAG1158.81495E-710.7399475
CCTAAGA803.765353E-410.6897172
GTCTTAT1355.574475E-810.5706491
GAACAAA7750.010.3114981
TAACACC1201.5196092E-610.2943824
CTGATTA1502.4539986E-810.1314419
CCAGTAC1356.428945E-79.8538963
CGCAAAA1451.6134436E-79.8296262
ACGCAAA1553.977766E-89.8204741
GCAAGAC1554.053618E-89.8084863
GTACATA4900.09.7077391
CAGTACT2350.09.7046884
AAAAGTA9250.09.6560582
Produced by FastQC (version 0.11.5)