FastQCFastQC Report
Wed 25 May 2016
SRR1294898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666040
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1704006.3915020029706975No Hit
GTACATGGGAAGCAGTGGTATCAAC1563195.863340384990473No Hit
GAGTACATGGGAAGCAGTGGTATCA681362.555700589638565No Hit
CATGTACTCTGCGTTGATACCACTG577992.1679719734137524No Hit
GCGTTGATACCACTGCTTCCCATGT523191.9624236695623472No Hit
GTATCAACGCAGAGTACATGGGAAG430811.6159172405515294No Hit
ACGCAGAGTACATGGGAAGCAGTGG394921.4812981050546878No Hit
TATCAACGCAGAGTACATGGGAAGC381501.4309612758998365No Hit
ACTCTGCGTTGATACCACTGCTTCC355551.3336259020869905No Hit
GCTTCCCATGTACTCTGCGTTGATA306831.1508829574950115No Hit
GGTATCAACGCAGAGTACATGGGAA295421.1080854000690163No Hit
ATACCACTGCTTCCCATGTACTCTG207280.7774827084364826No Hit
GTACTCTGCGTTGATACCACTGCTT204680.7677304166479122No Hit
CAGTGGTATCAACGCAGAGTACATG186380.6990892859822059No Hit
GGGAAGCAGTGGTATCAACGCAGAG185130.6944006841607778No Hit
GTGGTATCAACGCAGAGTACATGGG178440.6693072872124949No Hit
CATGGGAAGCAGTGGTATCAACGCA175720.6591048896490675No Hit
CTGCTTCCCATGTACTCTGCGTTGA172320.6463518926947832No Hit
TACCACTGCTTCCCATGTACTCTGC164330.6163823498522153No Hit
GCAGTGGTATCAACGCAGAGTACAT163540.6134191535010727No Hit
CCATGTACTCTGCGTTGATACCACT160300.6012662975799313No Hit
GCAGAGTACATGGGAAGCAGTGGTA150690.5652203267767926No Hit
GATACCACTGCTTCCCATGTACTCT148320.5563307377233649No Hit
ACATGGGAAGCAGTGGTATCAACGC137750.5166839207213695No Hit
CCACTGCTTCCCATGTACTCTGCGT135740.5091446489925132No Hit
CTTCCCATGTACTCTGCGTTGATAC133790.5018304301510855No Hit
GTTGATACCACTGCTTCCCATGTAC124520.46705975904337516No Hit
GTATCAACGCAGAGTACTTTTTTTT122070.4578700994733762No Hit
ATGGGAAGCAGTGGTATCAACGCAG116640.43750281316109285No Hit
AAGCAGTGGTATCAACGCAGAGTAC113860.4270753627102369No Hit
CCCCATGTACTCTGCGTTGATACCA105760.39669322290738324No Hit
ATCAACGCAGAGTACATGGGAAGCA93830.35194520712367405No Hit
TTCCCATGTACTCTGCGTTGATACC91080.34163028311653243No Hit
TGATACCACTGCTTCCCATGTACTC83030.3114356873865359No Hit
GGTATCAACGCAGAGTACTTTTTTT78700.29519437067710913No Hit
TATCAACGCAGAGTACTTTTTTTTT77350.29013068070996684No Hit
TCCATGTACTCTGCGTTGATACCAC70370.2639495281391127No Hit
GGAAGCAGTGGTATCAACGCAGAGT62780.23548033787940165No Hit
GTACATGGAAAGCAGTGGTATCAAC59420.22287737618340311No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55040.20644851540111928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA53450.2004846138842628No Hit
TGGGAAGCAGTGGTATCAACGCAGA49030.18390571784369325No Hit
TCCCATGTACTCTGCGTTGATACCA48400.18154266252569354No Hit
GTACATGGGGAGCAGTGGTATCAAC46990.17625391967112272No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46130.17302816161798024No Hit
AAAAAGTACTCTGCGTTGATACCAC45480.17059008867083764No Hit
GCTTTCCATGTACTCTGCGTTGATA44510.16695173365740948No Hit
CTGCGTTGATACCACTGCTTCCCAT43280.16233814946512432No Hit
CTCTGCGTTGATACCACTGCTTCCC40540.15206073427255404No Hit
GAGTACATGGAAAGCAGTGGTATCA40500.15191069901426835No Hit
GTACATGGTAAGCAGTGGTATCAAC38410.1440713567688407No Hit
TCAACGCAGAGTACATGGGAAGCAG38020.14260851300055513No Hit
ACCATGTACTCTGCGTTGATACCAC35420.13285622121198482No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34740.13030562182112798No Hit
GTACATGGGAAAGCAGTGGTATCAA33040.12392912334398584No Hit
GCGTTGATACCACTGCTTTCCATGT32980.1237040704565573No Hit
AAAGTACTCTGCGTTGATACCACTG31260.1172525543502723No Hit
ATGGAAAGCAGTGGTATCAACGCAG31050.11646486924427239No Hit
ACTCTGCGTTGATACCACTGCTTTC28700.1076502978199877No Hit
ACTGCTTCCCATGTACTCTGCGTTG27680.10382439873370242No Hit
GAGTAAGCAGTGGTATCAACGCAGA27270.10228653733627402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA5650.013.2910591
CCGGAAT550.003068755812.0912673
CTACACT1501.4551915E-1112.03368954
GTATTAG1701.8189894E-1211.74224951
CGCAAAA1157.068775E-811.5666462
AAAAGTA8750.011.2928222
CCCCTAT855.293894E-511.1830941
GTTCTAT909.4630035E-510.5618111
AAAAAGT9100.010.5502051
TCAGTAC1451.4440957E-810.4830693
GTATAAA2001.8189894E-1210.4561941
CGGAGTA750.002647983410.1336344
CCAGGAC3100.010.1132033
TATAGGA856.588759E-410.0600652
GTTACAC1054.1058673E-59.9526763
TGTACTG1252.570603E-69.8802935
TAATCCT1252.570603E-69.8802934
GTCTAGG1356.395203E-79.8576911
GTCTATA1002.740144E-49.50563051
GGTCTAC800.0044952169.50563051
Produced by FastQC (version 0.11.5)