FastQCFastQC Report
Wed 25 May 2016
SRR1294897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300596
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC106860.24847718781303801No Hit
CCCATGTACTCTGCGTTGATACCAC96620.22466653459194957No Hit
GTATCAACGCAGAGTACTTTTTTTT46110.10721769726800658No Hit
GAGTACATGGGAAGCAGTGGTATCA45870.10665963508313732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG2050.013.9038032
GCCGCGG1900.012.0011773
AGCCTCG3200.011.2823552
CGGCGGT700.001494153810.8555547
CCTCGGT1356.4384403E-79.8532764
AAGCCGC900.00110015129.5133341
ATACCGT1401.0619806E-69.498616
CGGCCGC800.00452364849.4986097
CTATACT5950.09.2618434
CTCGGCG850.0074403378.9410125
AGGACCG1201.765449E-48.7081745
GTATTAA9300.08.69498351
TGGACGG1000.00290704458.5498435
GTACTAA4350.08.3104991
CCGCGGC1150.00110965648.2620644
CGCGTGG1150.00111324258.25937211
GTCTTAG5650.08.2505021
TATACTG9750.08.1844645
GTCTAAG3850.08.1542861
CTCGGTC1050.00451594648.1427075
Produced by FastQC (version 0.11.5)