FastQCFastQC Report
Wed 25 May 2016
SRR1294897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300596
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116010.2697533086111785No Hit
CCCATGTACTCTGCGTTGATACCAC107750.2505466684152615No Hit
GTACATGGGAAGCAGTGGTATCAAC106160.24684950644050266No Hit
GGTATCAACGCAGAGTACTTTTTTT67840.15774557758971083No Hit
TATCAACGCAGAGTACTTTTTTTTT63470.14758419530688305No Hit
GAGTACATGGGAAGCAGTGGTATCA46220.10747347576940498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG655.4551398E-513.1542885
TAGACGT600.00587704711.0837074
CGTGCGT700.001493214710.85637210
CCGAATT1106.057222E-610.36290114
AGCCGTG951.6465357E-49.999297
CAGTGCG1002.7593484E-49.4993259
TTATACT8100.08.913884
GTCCTAA4400.08.6409751
AAGACGG1000.00290567788.5502875
GTATTAG6250.08.3644641
AGGCCCG1252.7442307E-48.3602815
ACCGTGA1252.7473326E-48.3594068
ATTAGAC4600.08.26143553
TAAGACT6100.08.2543764
GCACCGT1502.5871743E-58.233616
GTATAGG4750.08.2043781
CTAACGT1050.00451383378.1431314
TAAGACG1401.0394907E-48.1431314
CTAGGAC3650.08.0690463
TAGACAG7150.07.9722965
Produced by FastQC (version 0.11.5)