FastQCFastQC Report
Wed 25 May 2016
SRR1294896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045843
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC90230.22301903459921701No Hit
CCCATGTACTCTGCGTTGATACCAC84450.20873276595260865No Hit
GTATCAACGCAGAGTACTTTTTTTT47160.1165640881269985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGT909.5440126E-510.553248
ACACCGC1002.4068024E-510.4477066
GCGTAGC750.002646350310.1346212
AGGTCGT750.002653966210.131116
TCCGTAC900.00111656419.4979158
GTTCTAG4250.09.4024591
GTAGGAC3500.09.2297443
CGTAGAC1054.4897047E-49.0487683
CGGAGCG950.00182266529.0011433
CAGGACT5250.08.8682324
AAGACCG1304.369524E-58.7696935
TAGGACA5400.08.6218924
GTCCTAG4650.08.5936461
TTAGACT4100.08.5746844
TAACCCG1000.00290509768.5504515
GTCTTAG4600.08.480221
TCTATAC6150.08.3425233
GGCGCGA1150.00111212788.26018115
CTACACT6450.08.2495184
TAGGACT6000.08.2347874
Produced by FastQC (version 0.11.5)