FastQCFastQC Report
Wed 25 May 2016
SRR1294896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4045843
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112360.27771715313718304No Hit
GTACATGGGAAGCAGTGGTATCAAC91340.22576259138083213No Hit
CCCATGTACTCTGCGTTGATACCAC86960.21493666462094552No Hit
GGTATCAACGCAGAGTACTTTTTTT61580.15220560956023257No Hit
TATCAACGCAGAGTACTTTTTTTTT60150.1486711174902239No Hit
GAGTACATGGGAAGCAGTGGTATCA40750.10072066563136532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGG400.00528382114.24886910
ATACGAT550.003068890712.0914446
CGGTCGT600.005881891611.08245410
TATTCCG1502.1354936E-910.7671425
CCGGACC803.77195E-410.6879734
AGAACCG803.77195E-410.6879735
TAATACG1002.399812E-510.4504624
CTACGCT856.5951317E-410.0592684
TAGACAG6350.010.0240665
CTAGGAC4500.09.9226613
GTCTAAG3400.09.50558951
CTAAGAC3700.09.500423
TCTGTCG2106.730261E-119.4992478
GTCTTAG3300.09.2175421
GTTCTAG4650.09.1989571
TACACCG1554.2628562E-79.1939555
ACCGCTC950.00182609268.9992868
TCTACGC850.00743636078.9415713
TAGACTG7100.08.8313765
AACCGTG1304.3763117E-58.7685357
Produced by FastQC (version 0.11.5)